BMRB: 10031

Solution structure of the 2nd mbt domain from human KIAA1617 protein

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

KIAA1617 protein

Entity

1. KIAA1617 protein (polymer, Thiol state: all free), 127 monomers, 14439.17 Da Detail

GSSGSSGPID LITVGSLIEL QDSQNPFQYW IVSVIENVGG RLRLRYVGLE DTESYDQWLF YLDYRLRPVG WCQENKYRMD PPSEIYPLKM ASEWKCTLEK SLIDAAKFPL PMEVFKDHAD LSGPSSG

Formula weight

14439.17 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 94.5 %, Completeness (bb): 93.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.5 % (1421 of 1503)	94.0 % (737 of 784)	95.1 % (560 of 589)	95.4 % (124 of 130)
Backbone	93.7 % (697 of 744)	94.1 % (240 of 255)	93.0 % (345 of 371)	94.9 % (112 of 118)
Sidechain	94.3 % (826 of 876)	93.0 % (492 of 529)	96.4 % (323 of 335)	91.7 % (11 of 12)
Aromatic	97.3 % (144 of 148)	97.3 % (72 of 74)	97.1 % (68 of 70)	100.0 % (4 of 4)
Methyl	96.8 % (122 of 126)	96.8 % (61 of 63)	96.8 % (61 of 63)

1. mbt domain

GSSGSSGPID LITVGSLIEL QDSQNPFQYW IVSVIENVGG RLRLRYVGLE DTESYDQWLF YLDYRLRPVG WCQENKYRMD PPSEIYPLKM ASEWKCTLEK SLIDAAKFPL PMEVFKDHAD LSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	mbt domain	[U-13C; U-15N]		1.14 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.19 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WJR, Strand ID: A Detail

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Release date

2008-08-12

Citation

Solution structure of the 2nd mbt domain from human KIAA1617 protein
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. KIAA1617 protein, : 1 : 2 : 63 entities Detail

Experiments performed 2 experiments Detail