BMRB: 10034

Solution structure of Fibronectin type III domain derived from human KIAA0970 protein

Authors

Kobayashi, N., Koshiba, S., Inoue, M., Hayashi, F., Kigawa, T., Yokoyama, S.

Assembly

KIAA0970 protein

Entity

1. KIAA0970 protein (polymer, Thiol state: all free), 137 monomers, 14557.86 Da Detail

GSSGSSGDEE TKAFEALLSN IVKPVASDIQ ARTVVLTWSP PSSLINGETD ESSVPELYGY EVLISSTGKD GKYKSVYVGE ETNITLNDLK PAMDYHAKVQ AEYNSIKGTP SEAEIFTTLS CEPDIPNPPR ISGPSSG

Formula weight

14557.86 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 98.2 %, Completeness (bb): 97.9 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	98.2 % (1472 of 1499)	98.3 % (761 of 774)	98.1 % (579 of 590)	97.8 % (132 of 135)
Backbone	97.9 % (783 of 800)	98.2 % (269 of 274)	97.8 % (391 of 400)	97.6 % (123 of 126)
Sidechain	98.7 % (814 of 825)	98.4 % (492 of 500)	99.1 % (313 of 316)	100.0 % (9 of 9)
Aromatic	100.0 % (84 of 84)	100.0 % (42 of 42)	100.0 % (41 of 41)	100.0 % (1 of 1)
Methyl	100.0 % (144 of 144)	100.0 % (72 of 72)	100.0 % (72 of 72)

1. Fibronectin type3 domain

GSSGSSGDEE TKAFEALLSN IVKPVASDIQ ARTVVLTWSP PSSLINGETD ESSVPELYGY EVLISSTGKD GKYKSVYVGE ETNITLNDLK PAMDYHAKVQ AEYNSIKGTP SEAEIFTTLS CEPDIPNPPR ISGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	fibronectin type III domain	[U-13C; U-15N]		1.11 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.37 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1WK0, Strand ID: A Detail

---

Release date

2008-08-12

Citation

Solution structure of Fibronectin type III domain derived from human KIAA0970 protein
Kobayashi, N., Koshiba, S., Inoue, M., Hayashi, F., Kigawa, T., Yokoyama, S.

Related entities 1. KIAA0970 protein, : 1 : 54 entities Detail

Interaction partners 1. KIAA0970 protein, : 27 interactors Detail

Experiments performed 2 experiments Detail