BMRB: 10040

Solution Structure of the RA Domain of Human Grb7 Protein

Authors

Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Growth factor receptor-bound protein 7

Entity

1. Growth factor receptor-bound protein 7 (polymer, Thiol state: all free), 100 monomers, 10613.63 Da Detail

GSSGSSGRPH VVKVYSEDGA CRSVEVAAGA TARHVCEMLV QRAHALSDET WGLVECHPHL ALERGLEDHE SVVEVQAAWP VGGDSRFVFR KNFASGPSSG

Formula weight

10613.63 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.0 %, Completeness: 93.7 %, Completeness (bb): 91.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.7 % (993 of 1060)	92.8 % (503 of 542)	94.7 % (395 of 417)	94.1 % (95 of 101)
Backbone	91.7 % (543 of 592)	89.4 % (185 of 207)	92.7 % (268 of 289)	93.8 % (90 of 96)
Sidechain	96.1 % (535 of 557)	94.9 % (318 of 335)	97.7 % (212 of 217)	100.0 % (5 of 5)
Aromatic	97.7 % (84 of 86)	97.7 % (42 of 43)	97.6 % (40 of 41)	100.0 % (2 of 2)
Methyl	98.0 % (100 of 102)	96.1 % (49 of 51)	100.0 % (51 of 51)

1. RA domain

GSSGSSGRPH VVKVYSEDGA CRSVEVAAGA TARHVCEMLV QRAHALSDET WGLVECHPHL ALERGLEDHE SVVEVQAAWP VGGDSRFVFR KNFASGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	RA domain	[U-13C, U-15N]		1.17 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WGR, Strand ID: A Detail

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Release date

2008-08-12

Citation

Solution Structure of the RA Domain of Human Grb7 Protein
Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Growth factor receptor-bound protein 7, : 1 : 40 entities Detail

Interaction partners 1. Growth factor receptor-bound protein 7, : 45 interactors Detail

Experiments performed 2 experiments Detail