BMRB: 10042

Solution Structure of the CS Domain of Human KIAA1068 Protein

Authors

Li, H., Hayashi, F., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

KIAA1068 protein

Entity

1. KIAA1068 protein (polymer, Thiol state: all free), 124 monomers, 13522.89 Da Detail

GSSGSSGQKN PDSYNGAVRE NYTWSQDYTD LEVRVPVPKH VVKGKQVSVA LSSSSIRVAM LEENGERVLM EGKLTHKINT ESSLWSLEPG KCVLVNLSKV GEYWWNAILE GEEPIDIDSG PSSG

Formula weight

13522.89 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.0 %, Completeness: 96.1 %, Completeness (bb): 94.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.1 % (1340 of 1394)	96.0 % (695 of 724)	97.2 % (523 of 538)	92.4 % (122 of 132)
Backbone	94.9 % (695 of 732)	94.5 % (240 of 254)	96.1 % (346 of 360)	92.4 % (109 of 118)
Sidechain	97.3 % (753 of 774)	96.8 % (455 of 470)	98.3 % (285 of 290)	92.9 % (13 of 14)
Aromatic	98.9 % (87 of 88)	100.0 % (44 of 44)	97.5 % (39 of 40)	100.0 % (4 of 4)
Methyl	100.0 % (128 of 128)	100.0 % (64 of 64)	100.0 % (64 of 64)

1. CS domain

GSSGSSGQKN PDSYNGAVRE NYTWSQDYTD LEVRVPVPKH VVKGKQVSVA LSSSSIRVAM LEENGERVLM EGKLTHKINT ESSLWSLEPG KCVLVNLSKV GEYWWNAILE GEEPIDIDSG PSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	CS domain of Human KIAA1068 protein	[U-13C, U-15N]		1.06 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	2 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.63 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WGV, Strand ID: A Detail

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Release date

2008-08-12

Citation

Solution Structure of the CS Domain of Human KIAA1068 Protein
Li, H., Hayashi, F., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. KIAA1068 protein, : 1 : 3 : 51 entities Detail

Interaction partners 1. KIAA1068 protein, : 123 interactors Detail

Experiments performed 2 experiments Detail