BMRBj - BMREntryMaster

Found 1 Document (1.161 seconds)

BMRB: 10051

Backbone 1H and 15N assignment for the human c-Ha Ras (Y32W) in the GDP-bound state

Authors

Matsuda, T., Koshiba, S., Tochio, N., Kigawa, T., Yokoyama, S.

Assembly

RasY32W

Entity

1. RasY32W (polymer, Thiol state: all free), 178 monomers, 20034.33 Da Detail

GSSGSSGMTE YKLVVVGAGG VGKSALTIQL IQNHFVDEWD PTIEDSYRKQ VVIDGETCLL DILDTAGQEE YSAMRDQYMR TGEGFLCVFA INNTKSFEDI HQYREQIKRV KDSDDVPMVL VGNKCDLAAR TVESRQAQDL ARSYGIPYIE TSAKTRQGVE DAFYTLVREI RQHKLRKL

2. GDP (non-polymer), 443.201 Da

Total weight

20477.531 Da

Max. entity weight

20034.33 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.6 %, Completeness: 31.0 %, Completeness (bb): 65.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	31.0 % (389 of 1253)	20.4 % (217 of 1062)	90.1 % (172 of 191)
Backbone	65.5 % (355 of 542)	49.9 % (183 of 367)	98.3 % (172 of 175)
Sidechain	4.8 % (34 of 711)	4.9 % (34 of 695)	0.0 % (0 of 16)
Aromatic	0.0 % (0 of 70)	0.0 % (0 of 69)	0.0 % (0 of 1)
Methyl	8.0 % (8 of 100)	8.0 % (8 of 100)

1. RasY32W

GSSGSSGMTE YKLVVVGAGG VGKSALTIQL IQNHFVDEWD PTIEDSYRKQ VVIDGETCLL DILDTAGQEE YSAMRDQYMR TGEGFLCVFA INNTKSFEDI HQYREQIKRV KDSDDVPMVL VGNKCDLAAR TVESRQAQDL ARSYGIPYIE TSAKTRQGVE DAFYTLVREI RQHKLRKL

Show sample condition

Sample

Pressure 1 (±0.03) atm, Temperature 298 (±0.1) K, pH 6.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	RasY32W	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	5 mM
5	magnesium chloride		salt	5 mM
6	D2O		solvent	10 %
7	H2O		solvent	90 %
8	sodium azide		salt	0.01 %

Release date

2007-11-12

Citation

Improving cell-free protein synthesis for stable-isotope labeling
Matsuda, T., Koshiba, S., Tochio, N., Seki, E., Iwasaki, N., Yabuki, T., Inoue, M., Yokoyama, S., Kigawa, T.
J. Biomol. NMR (2007), 37, 225-229, PubMed 17237976 , DOI 10.1007/s10858-006-9127-5 , Abstract

Related entities 1. RasY32W, : 300 entities Detail

Interaction partners 1. RasY32W, : 62 interactors Detail

More details for 62 interactors identified by 29 citations

Experiments performed 1 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr10051_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr10051_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shift_1	Warning	344		96.6 (chain: 1, length: 178)
1	Chemical shift references	reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shift_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 178, Sequence coverage: 96.6%
- Total number of assignments
  - ¹H chemical shifts: 172
  - ¹⁵N chemical shifts: 172
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1062	172	16.2
¹⁵N chemical shifts	203	172	84.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	367	172	46.9
¹⁵N chemical shifts	175	172	98.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	695	0	0.0
¹⁵N chemical shifts	28	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	104	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	69	0	0.0
¹⁵N chemical shifts	1	0	0.0

Keywords Ras Y32W mutant

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Found 1 Document (1.161 seconds)

BMRB: 10051

Sample

Related entities 1. RasY32W, : 300 entities Detail

Interaction partners 1. RasY32W, : 62 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 4 contents Detail