BMRB: 10065

Solution structure of the SAND domain of the putative nuclear protein homolog (5830484A20Rik)

Authors

Tochio, N., Kobayashi, N., Koshiba, S., Kigawa, T., Inoue, M., Shirouzu, M., Terada, T., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Matsuo, Y., Arakawa, T., Carninci, P., Kawai, J., Hayashizaki, Y., Yokoyama, S.

Assembly

putative nuclear protein homolog 5830484A20Rik

Entity

1. putative nuclear protein homolog 5830484A20Rik (polymer, Thiol state: all free), 94 monomers, 9944.045 Da Detail

GSSGSSGNDA VDFSPTLPVT CGKAKGTLFQ EKLKQGASKK CIQNEAGDWL TVKEFLNEGG RATSKDWKGV IRCNGETLRH LEQKGLLFSG PSSG

Formula weight

9944.045 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 96.4 %, Completeness (bb): 96.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.4 % (1010 of 1048)	95.3 % (522 of 548)	97.7 % (390 of 399)	97.0 % (98 of 101)
Backbone	96.6 % (539 of 558)	94.5 % (188 of 199)	98.1 % (263 of 268)	96.7 % (88 of 91)
Sidechain	96.5 % (550 of 570)	95.7 % (334 of 349)	97.6 % (206 of 211)	100.0 % (10 of 10)
Aromatic	88.2 % (60 of 68)	85.3 % (29 of 34)	90.6 % (29 of 32)	100.0 % (2 of 2)
Methyl	100.0 % (82 of 82)	100.0 % (41 of 41)	100.0 % (41 of 41)

1. SAND DOMAIN

GSSGSSGNDA VDFSPTLPVT CGKAKGTLFQ EKLKQGASKK CIQNEAGDWL TVKEFLNEGG RATSKDWKGV IRCNGETLRH LEQKGLLFSG PSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SAND domain	[U-13C; U-15N]		1.3 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 %
5	H2O		solvent	90 %
6	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.01 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.01 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.15 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UFN, Strand ID: A Detail

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Release date

2008-08-13

Citation

Solution structure of the SAND domain of the putative nuclear protein homolog (5830484A20Rik)
Tochio, N., Kobayashi, N., Koshiba, S., Kigawa, T., Inoue, M., Shirouzu, M., Terada, T., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Matsuo, Y., Arakawa, T., Carninci, P., Kawai, J., Hayashizaki, Y., Yokoyama, S.

Related entities 1. putative nuclear protein homolog 5830484A20Rik, : 1 : 47 entities Detail

Experiments performed 2 experiments Detail