BMRB: 10067

Solution Structure of The FHA Domain of Arabidopsis thaliana Hypothetical Protein

Authors

Tomizawa, T., Inoue, M., Koshiba, S., Hayashi, F., Shirouzu, M., Terada, T., Yabuki, T., Aoki, M., Matsuda, T., Seki, E., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Shinozaki, K., Seki, M., Kigawa, T., Yokoyama, S.

Assembly

expressed protein

Entity

1. expressed protein (polymer, Thiol state: all free), 118 monomers, 12245.51 Da Detail

GSSGSSGMVT PSLRLVFVKG PREGDALDYK PGSTIRVGRI VRGNEIAIKD AGISTKHLRI ESDSGNWVIQ DLGSSNGTLL NSNALDPETS VNLGDGDVIK LGEYTSILVN FVSGPSSG

Formula weight

12245.51 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.5 %, Completeness: 96.1 %, Completeness (bb): 94.8 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.1 % (1219 of 1268)	95.7 % (625 of 653)	97.4 % (480 of 493)	93.4 % (114 of 122)
Backbone	94.8 % (662 of 698)	94.0 % (233 of 248)	96.1 % (324 of 337)	92.9 % (105 of 113)
Sidechain	97.6 % (655 of 671)	96.8 % (392 of 405)	98.8 % (254 of 257)	100.0 % (9 of 9)
Aromatic	100.0 % (52 of 52)	100.0 % (26 of 26)	100.0 % (25 of 25)	100.0 % (1 of 1)
Methyl	98.6 % (138 of 140)	97.1 % (68 of 70)	100.0 % (70 of 70)

1. Forkhead Associated Domain, FHA domain

GSSGSSGMVT PSLRLVFVKG PREGDALDYK PGSTIRVGRI VRGNEIAIKD AGISTKHLRI ESDSGNWVIQ DLGSSNGTLL NSNALDPETS VNLGDGDVIK LGEYTSILVN FVSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	FHA domain	[U-13C; U-15N]		1.09 mM
2	Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 %
5	H2O		solvent	90 %
6	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.21 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.21 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.15 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.66 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1UHT, Strand ID: A Detail

---

Release date

2008-08-13

Citation

Solution Structure of The FHA Domain of Arabidopsis thaliana Hypothetical Protein
Tomizawa, T., Inoue, M., Koshiba, S., Hayashi, F., Shirouzu, M., Terada, T., Yabuki, T., Aoki, M., Matsuda, T., Seki, E., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Shinozaki, K., Seki, M., Kigawa, T., Yokoyama, S.

Related entities 1. expressed protein, : 1 : 2 : 35 entities Detail

Interaction partners 1. expressed protein, : 2 interactors Detail

Experiments performed 2 experiments Detail