BMRB: 10069

Solution structure of the Villin headpiece domain of human actin-binding LIM protein homologue (KIAA0843 protein)

Authors

Tochio, N., Koshiba, S., Kigawa, T., Yokoyama, S.

Assembly

actin-binding LIM protein homologue

Entity

1. actin-binding LIM protein homologue (polymer, Thiol state: all free), 88 monomers, 10227.45 Da Detail

GSSGSSGNAV NWGMREYKIY PYELLLVTTR GRNRLPKDVD RTRLERHLSQ EEFYQVFGMT ISEFDRLALW KRNELKKQAR LFSGPSSG

Formula weight

10227.45 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 96.6 %, Completeness (bb): 97.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.6 % (1025 of 1061)	95.9 % (537 of 560)	97.3 % (396 of 407)	97.9 % (92 of 94)
Backbone	97.1 % (507 of 522)	95.6 % (173 of 181)	98.0 % (251 of 256)	97.6 % (83 of 85)
Sidechain	96.4 % (597 of 619)	96.0 % (364 of 379)	97.0 % (224 of 231)	100.0 % (9 of 9)
Aromatic	98.0 % (98 of 100)	96.0 % (48 of 50)	100.0 % (48 of 48)	100.0 % (2 of 2)
Methyl	94.9 % (74 of 78)	94.9 % (37 of 39)	94.9 % (37 of 39)

1. VHP domain

GSSGSSGNAV NWGMREYKIY PYELLLVTTR GRNRLPKDVD RTRLERHLSQ EEFYQVFGMT ISEFDRLALW KRNELKKQAR LFSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	VHP domain	[U-13C; U-15N]		1.2 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 %
5	H2O		solvent	90 %
6	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.52 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UJS, Strand ID: A Detail

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Release date

2008-08-13

Citation

Solution structure of the Villin headpiece domain of human actin-binding LIM protein homologue (KIAA0843 protein)
Tochio, N., Koshiba, S., Kigawa, T., Yokoyama, S.

Related entities 1. actin-binding LIM protein homologue, : 1 : 2 : 29 entities Detail

Interaction partners 1. actin-binding LIM protein homologue, : 12 interactors Detail

Experiments performed 2 experiments Detail