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LPS bound structure of Temporin-1Tl
Authors
Bhattacharyya, S.
Assembly
TemporinL
Entity
1. TemporinL (polymer, Thiol state: not present), 13 monomers, 1640.966 × 2 Da Detail

FVQWFSKFLG RIL


Total weight
3281.932 Da
Max. entity weight
1640.966 Da
Source organism
Rana temporaria
Exptl. method
SOLUTION NMR
Data set
assigned_chemical_shifts, conformer_family_coord_set
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 84.5 %, Completeness (bb): 96.2 % Detail

Polymer type: polypeptide(L)

Total1H
All84.5 % (82 of 97)84.5 % (82 of 97)
Backbone96.2 % (25 of 26)96.2 % (25 of 26)
Sidechain80.3 % (57 of 71)80.3 % (57 of 71)
Aromatic66.7 % (14 of 21)66.7 % (14 of 21)
Methyl100.0 % (8 of 8)100.0 % (8 of 8)

1. entity

FVQWFSKFLG RIL

Sample

Solvent system 90% H2O/10% D2O, Temperature 290 K, pH 4.5, Details 0.5mM TemporinL and 14.5micro molar of E.coli LPS


#NameIsotope labelingTypeConcentration
1TemporinLnatural abundance0.5 mM
2LPSnatural abundance14.5 mM
3H20natural abundance90 %
4D20natural abundance10 %

Release date
2011-07-10
Citation
NMR structures and interactions of temporin-1Tl and temporin-1Tb with lipopolysaccharide micelles: mechanistic insights into outer membrane permeabilization and synergistic activity
Bhunia, A., Saravanan, R., Mohanram, H., Mangoni, M.L., Bhattacharjya, S.
J. Biol. Chem. (2011), 286, 24394-24406, PubMed 21586570 , DOI 10.1074/jbc.M110.189662 ,
Related entities 1. TemporinL, : 1 entities Detail
Experiments performed 2 experiments Detail
Keywords antimicrobial peptide