BMRB: 10114

Solution Structure of the C-terminal Pleckstrin Homology Domain of Sbf1 from Mouse

Authors

Li, H., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

SET binding factor 1

Entity

1. SET binding factor 1 (polymer, Thiol state: all free), 117 monomers, 13039.60 Da Detail

GSSGSSGRSY EGILYKKGAF MKPWKARWFV LDKTKHQLRY YDHRMDTECK GVIDLAEVEA VAPGTPTIGA PKTVDEKAFF DVKTTRRVYN FCAQDVPSAQ QWVDRIQSCL SSGPSSG

Formula weight

13039.6 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.4 %, Completeness (bb): 97.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.4 % (1301 of 1350)	95.4 % (671 of 703)	97.0 % (511 of 527)	99.2 % (119 of 120)
Backbone	97.5 % (673 of 690)	95.8 % (228 of 238)	98.2 % (335 of 341)	99.1 % (110 of 111)
Sidechain	95.6 % (733 of 767)	95.3 % (443 of 465)	95.9 % (281 of 293)	100.0 % (9 of 9)
Aromatic	92.5 % (124 of 134)	92.5 % (62 of 67)	92.2 % (59 of 64)	100.0 % (3 of 3)
Methyl	100.0 % (104 of 104)	100.0 % (52 of 52)	100.0 % (52 of 52)

1. The Pleckstrin Homology Domain

GSSGSSGRSY EGILYKKGAF MKPWKARWFV LDKTKHQLRY YDHRMDTECK GVIDLAEVEA VAPGTPTIGA PKTVDEKAFF DVKTTRRVYN FCAQDVPSAQ QWVDRIQSCL SSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Pleckstrin Homology domain	[U-13C; U-15N]		0.28 mM
2	PiNa			20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.19 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.19 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.32 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1V5U, Strand ID: A Detail

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Release date

2008-08-14

Citation

Solution Structure of the C-terminal Pleckstrin Homology Domain of Sbf1 from Mouse
Li, H., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. SET binding factor 1, : 1 : 208 entities Detail

Interaction partners 1. SET binding factor 1, : 5 interactors Detail

Experiments performed 2 experiments Detail