BMRB: 10131

Solution Structure of the N-terminal Pleckstrin Homology Domain Of TAPP2 from Mouse

Authors

Li, H., Hayashi, F., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

pleckstrin homology domain-containing, family A

Entity

1. pleckstrin homology domain-containing, family A (polymer, Thiol state: all free), 126 monomers, 13916.55 Da Detail

GSSGSSGMPY VDRQNRICGF LDIEDNENSG KFLRRYFILD TQANCLLWYM DNPQNLAVGA GAVGSLQLTY ISKVSIATPK QKPKTPFCFV INALSQRYFL QANDQKDLKD WVEALNQASK SGPSSG

Formula weight

13916.55 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.8 %, Completeness: 95.6 %, Completeness (bb): 94.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.6 % (1400 of 1464)	94.6 % (723 of 764)	96.8 % (542 of 560)	96.4 % (135 of 140)
Backbone	94.9 % (706 of 744)	94.1 % (241 of 256)	95.1 % (350 of 368)	95.8 % (115 of 120)
Sidechain	96.3 % (805 of 836)	94.9 % (482 of 508)	98.4 % (303 of 308)	100.0 % (20 of 20)
Aromatic	97.6 % (121 of 124)	95.2 % (59 of 62)	100.0 % (60 of 60)	100.0 % (2 of 2)
Methyl	100.0 % (122 of 122)	100.0 % (61 of 61)	100.0 % (61 of 61)

1. Pleckstrin Homology domain

GSSGSSGMPY VDRQNRICGF LDIEDNENSG KFLRRYFILD TQANCLLWYM DNPQNLAVGA GAVGSLQLTY ISKVSIATPK QKPKTPFCFV INALSQRYFL QANDQKDLKD WVEALNQASK SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Pleckstrin Homology domain	[U-13C; U-15N]		1.08 mM
2	PiNa			20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.26 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1V5P, Strand ID: A Detail

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Release date

2008-09-01

Citation

Solution Structure of the N-terminal Pleckstrin Homology Domain Of TAPP2 from Mouse
Li, H., Hayashi, F., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.
Not known

Related entities 1. pleckstrin homology domain-containing, family A, : 1 : 3 : 69 entities Detail

Interaction partners 1. pleckstrin homology domain-containing, family A, : 1 interactors Detail

Experiments performed 2 experiments Detail