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1H and 15N assignment of oxidized Pseudomonas aeruginosa cytochrome c551
Authors
Takayama, S.J., Takahashi, Y., Mikami, S., Irie, K., Kawano, S., Yamamoto, Y., Hemmi, H., Kitahara, R., Yokoyama, S., Akasaka, K.
Assembly
cytochrome c551
Entity
1. cytochrome c551 (polymer, Thiol state: all other bound), 82 monomers, 8694.811 Da Detail

EDPEVLFKNK GCVACHAIDT KMVGPAYKDV AAKFAGQAGA EAELAQRIKN GSQGVWGPIP MPPNAVSDDE AQTLAKWVLS QK


2. HEC (non-polymer), 618.503 Da
Total weight
9313.313 Da
Max. entity weight
8694.811 Da
Entity Connection
thioether 2, metal coordination 2 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1thioethersing1:CYS12:SG2:HEC1:CAB
2thioethersing1:CYS15:SG2:HEC1:CAC
3metal coordinationsing1:HIS16:NE2:HEC1:FE
4metal coordinationsing1:MET61:SD2:HEC1:FE

Source organism
Pseudomonas aeruginosa
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 90.2 %, Completeness: 29.3 %, Completeness (bb): 62.4 % Detail

Polymer type: polypeptide(L)

Total1H15N
All29.3 % (165 of 564)19.1 % (91 of 477)85.1 % (74 of 87)
Backbone62.4 % (146 of 234)45.6 % (72 of 158)97.4 % (74 of 76)
Sidechain 5.8 % (19 of 330) 6.0 % (19 of 319) 0.0 % (0 of 11)
Aromatic 0.0 % (0 of 30) 0.0 % (0 of 28) 0.0 % (0 of 2)
Methyl 9.3 % (4 of 43) 9.3 % (4 of 43)

1. cytochrome c551

EDPEVLFKNK GCVACHAIDT KMVGPAYKDV AAKFAGQAGA EAELAQRIKN GSQGVWGPIP MPPNAVSDDE AQTLAKWVLS QK

Sample

Temperature 296 (±2) K, pH 6.0 (±0.2)


#NameIsotope labelingTypeConcentration
1cytochrome c551[U-15N]1 mM
2potassium phosphate20 mM
3potassium ferricyanide10 mM

Protein Blocks Logo
Calculated from 10 models in PDB: 2PAC, Strand ID: A Detail


Release date
2008-02-10
Citation
Local conformational transition of Hydrogenobacter thermophilus cytochrome c552 relevant to its redox potential
Takayama, S.J., Takahashi, Y., Mikami, S., Irie, K., Kawano, S., Yamamoto, Y., Hemmi, H., Kitahara, R., Yokoyama, S., Akasaka, K.
Biochemistry (2007), 46, 9215-9224, PubMed 17658890 , DOI 10.1021/bi7000714 ,
Related entities 1. cytochrome c551, : 1 : 4 : 1 : 49 entities Detail
Experiments performed 1 experiments Detail
Keywords Cytochrome c, High-pressure NMR, Hydrogen bond, Paramagnetic NMR, Redox potential