BMRB: 10133

1H and 15N assignment of reduced Pseudomonas aeruginosa cytochrome c551

Authors

Takayama, S.J., Takahashi, Y., Mikami, S., Irie, K., Kawano, S., Yamamoto, Y., Hemmi, H., Kitahara, R., Yokoyama, S., Akasaka, K.

Assembly

cytochrome c551

Entity

1. cytochrome c551 (polymer, Thiol state: all other bound), 82 monomers, 8694.811 Da Detail

EDPEVLFKNK GCVACHAIDT KMVGPAYKDV AAKFAGQAGA EAELAQRIKN GSQGVWGPIP MPPNAVSDDE AQTLAKWVLS QK

2. HEC (non-polymer), 618.503 Da

Total weight

9313.313 Da

Max. entity weight

8694.811 Da

Entity Connection

thioether 2, metal coordination 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	thioether	sing	1:CYS12:SG	2:HEC1:CAB
2	thioether	sing	1:CYS15:SG	2:HEC1:CAC
3	metal coordination	sing	1:HIS16:NE	2:HEC1:FE
4	metal coordination	sing	1:MET61:SD	2:HEC1:FE

Source organism

Pseudomonas aeruginosa

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.2 %, Completeness: 26.8 %, Completeness (bb): 61.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	26.8 % (151 of 563)	16.1 % (77 of 477)	86.0 % (74 of 86)
Backbone	61.8 % (149 of 241)	45.5 % (75 of 165)	97.4 % (74 of 76)
Sidechain	0.6 % (2 of 322)	0.6 % (2 of 312)	0.0 % (0 of 10)
Aromatic	0.0 % (0 of 30)	0.0 % (0 of 28)	0.0 % (0 of 2)
Methyl	2.3 % (1 of 43)	2.3 % (1 of 43)

1. cytochrome c551

EDPEVLFKNK GCVACHAIDT KMVGPAYKDV AAKFAGQAGA EAELAQRIKN GSQGVWGPIP MPPNAVSDDE AQTLAKWVLS QK

Show sample condition

Sample

Temperature 296 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	cytochrome c551	[U-15N]		1 mM
2	potassium phosphate			20 mM
3	sodium hydrosulfite			100 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 2PAC, Strand ID: A Detail

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Release date

2008-02-10

Citation

Local conformational transition of Hydrogenobacter thermophilus cytochrome c552 relevant to its redox potential
Takayama, S.J., Takahashi, Y., Mikami, S., Irie, K., Kawano, S., Yamamoto, Y., Hemmi, H., Kitahara, R., Yokoyama, S., Akasaka, K.
Biochemistry (2007), 46, 9215-9224, PubMed 17658890 , DOI 10.1021/bi7000714 , Abstract

Related entities 1. cytochrome c551, : 1 : 4 : 1 : 49 entities Detail

Experiments performed 1 experiments Detail