BMRB: 10140

Backbone NMR assignments of of RimM complexed with rS19

Authors

Suzuki, S., Tatsuguchi, A., Matsumoto, E., Kawazoe, M., Kaminishi, T., Shirouzu, M., Muto, Y., Takemoto, C., Yokoyama, S.

Assembly

RimM complexed to S19

Entity

1. RimM (polymer, Thiol state: not present), 162 monomers, 18072.56 Da Detail

MRLVEIGRFG APYALKGGLR FRGEPVVLHL ERVYVEGHGW RAIEDLYRVG EELVVHLAGV TDRTLAEALV GLRVYAEVAD LPPLEEGRYY YFALIGLPVY VEGRQVGEVV DILDAGAQDV LIIRGVGERL RDRAERLVPL QAPYVRVEEG SIHVDPIPGL FD

2. rS19 (polymer, Thiol state: not present), 93 monomers, 10581.26 Da Detail

MPRSLKKGVF VDDHLLEKVL ELNAKGEKRL IKTWSRRSTI VPEMVGHTIA VYNGKQHVPV YITENMVGHK LGEFAPTRTY RGHGKEAKAT KKK

Total weight

28653.82 Da

Max. entity weight

18072.56 Da

Source organism

Thermus thermophilus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 63.9 %, Completeness: 20.5 %, Completeness (bb): 33.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	20.5 % (614 of 2993)	15.3 % (238 of 1555)	19.3 % (229 of 1187)	58.6 % (147 of 251)
Backbone	33.4 % (502 of 1504)	31.1 % (163 of 524)	26.0 % (192 of 738)	60.7 % (147 of 242)
Sidechain	7.1 % (122 of 1717)	6.5 % (67 of 1031)	8.1 % (55 of 677)	0.0 % (0 of 9)
Aromatic	3.2 % (7 of 216)	3.7 % (4 of 108)	2.8 % (3 of 106)	0.0 % (0 of 2)
Methyl	12.3 % (42 of 342)	12.9 % (22 of 171)	11.7 % (20 of 171)

1. RimM

MRLVEIGRFG APYALKGGLR FRGEPVVLHL ERVYVEGHGW RAIEDLYRVG EELVVHLAGV TDRTLAEALV GLRVYAEVAD LPPLEEGRYY YFALIGLPVY VEGRQVGEVV DILDAGAQDV LIIRGVGERL RDRAERLVPL QAPYVRVEEG SIHVDPIPGL FD

2. rS19

MPRSLKKGVF VDDHLLEKVL ELNAKGEKRL IKTWSRRSTI VPEMVGHTIA VYNGKQHVPV YITENMVGHK LGEFAPTRTY RGHGKEAKAT KKK

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 318 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	RimM	[U-13C; U-15N]	protein	0.03 (±0.001) mM
2	rS19		protein	0.03 (±0.001) mM
3	HEPES		buffer	20 (±0.1) mM
4	D2O		solvent	10 (±0.1) %
5	H2O		solvent	90 (±0.1) %
6	NaCl		salt	300 (±1.0) mM

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Release date

2008-06-11

Citation

Structural characterization of the ribosome maturation protein, RimM
Suzuki, S., Tatsuguchi, A., Matsumoto, E., Kawazoe, M., Kaminishi, T., Shirouzu, M., Muto, Y., Takemoto, C., Yokoyama, S.
J. Bacteriol. (2007), 189, 6397-6406, PubMed 17616598 , DOI 10.1128/JB.00024-07 , Abstract

Related entities 1. RimM, : 1 : 2 : 1 : 82 entities Detail

Related entities 2. rS19, : 1 : 257 entities Detail

Experiments performed 3 experiments Detail

Chemical shift validation 4 contents Detail