BMRB: 10144

Solution structure of the RhoGAP domain from human Rho GTPase activating protein 5 variant

Authors

Tomizawa, T., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Rho GTPase activating protein 5 variant

Entity

1. Rho GTPase activating protein 5 variant (polymer, Thiol state: all free), 209 monomers, 23932.93 Da Detail

GSSGSSGWES NYFGMPLQDL VTAEKPIPLF VEKCVEFIED TGLCTEGLYR VSGNKTDQDN IQKQFDQDHN INLVSMEVTV NAVAGALKAF FADLPDPLIP YSLHPELLEA AKIPDKTERL HALKEIVKKF HPVNYDVFRY VITHLNRVSQ QHKINLMTAD NLSICFWPTL MRPDFENREF LSTTKIHQSV VETFIQQCQF FFYNGEIVE

Formula weight

23932.93 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 95.6 %, Completeness (bb): 95.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.6 % (2399 of 2510)	96.3 % (1253 of 1301)	95.0 % (937 of 986)	93.7 % (209 of 223)
Backbone	95.5 % (1176 of 1232)	96.4 % (401 of 416)	95.6 % (591 of 618)	92.9 % (184 of 198)
Sidechain	95.8 % (1416 of 1478)	96.3 % (852 of 885)	94.9 % (539 of 568)	100.0 % (25 of 25)
Aromatic	90.8 % (227 of 250)	93.6 % (117 of 125)	87.8 % (108 of 123)	100.0 % (2 of 2)
Methyl	97.9 % (231 of 236)	98.3 % (116 of 118)	97.5 % (115 of 118)

1. RhoGAP domain

GSSGSSGWES NYFGMPLQDL VTAEKPIPLF VEKCVEFIED TGLCTEGLYR VSGNKTDQDN IQKQFDQDHN INLVSMEVTV NAVAGALKAF FADLPDPLIP YSLHPELLEA AKIPDKTERL HALKEIVKKF HPVNYDVFRY VITHLNRVSQ QHKINLMTAD NLSICFWPTL MRPDFENREF LSTTKIHQSV VETFIQQCQF FFYNGEIVE

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	RhoGAP domain	[U-13C; U-15N]	protein	1.31 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EE4, Strand ID: A Detail

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Release date

2008-12-09

Citation

Solution structure of the RhoGAP domain from human Rho GTPase activating protein 5 variant
Tomizawa, T., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.
Not known

Related entities 1. Rho GTPase activating protein 5 variant, : 1 : 2 : 151 entities Detail

Interaction partners 1. Rho GTPase activating protein 5 variant, : 2 interactors Detail

Experiments performed 2 experiments Detail