BMRB: 10148

Solution structure of three zf-C2H2 domains from mouse protein odd-skipped-related 2 splicing isoform 2

Authors

Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Protein odd-skipped-related 2

Entity

1. zf-C2H2 domains (polymer, Thiol state: free and other bound), 106 monomers, 12196.75 Da Detail

GSSGSSGGRL PSKTKKEFIC KFCGRHFTKS YNLLIHERTH TDERPYTCDI CHKAFRRQDH LRDHRYIHSK EKPFKCQECG KGFCQSRTLA VHKTLHMQTS SPTAAS

2. ZN (non-polymer), 65.409 × 3 Da

Total weight

12392.977 Da

Max. entity weight

12196.75 Da

Entity Connection

na 12 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS20:SG	2:ZN1:ZN
2	na	sing	1:CYS23:SG	2:ZN1:ZN
3	na	sing	1:HIS36:NE2	2:ZN1:ZN
4	na	sing	1:HIS40:NE2	2:ZN1:ZN
5	na	sing	1:CYS48:SG	2:ZN1:ZN
6	na	sing	1:CYS51:SG	2:ZN1:ZN
7	na	sing	1:HIS64:NE2	2:ZN1:ZN
8	na	sing	1:HIS68:NE2	2:ZN1:ZN
9	na	sing	1:CYS76:SG	2:ZN1:ZN
10	na	sing	1:CYS79:SG	2:ZN1:ZN
11	na	sing	1:HIS92:NE2	2:ZN1:ZN
12	na	sing	1:HIS96:NE2	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.4 %, Completeness: 93.7 %, Completeness (bb): 91.1 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.7 % (1169 of 1247)	93.6 % (616 of 658)	95.2 % (459 of 482)	87.9 % (94 of 107)
Backbone	91.1 % (572 of 628)	89.8 % (193 of 215)	93.2 % (290 of 311)	87.3 % (89 of 102)
Sidechain	96.2 % (691 of 718)	95.5 % (423 of 443)	97.4 % (263 of 270)	100.0 % (5 of 5)
Aromatic	96.7 % (116 of 120)	96.7 % (58 of 60)	96.7 % (58 of 60)
Methyl	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (35 of 35)

1. zf-C2H2 domains

GSSGSSGGRL PSKTKKEFIC KFCGRHFTKS YNLLIHERTH TDERPYTCDI CHKAFRRQDH LRDHRYIHSK EKPFKCQECG KGFCQSRTLA VHKTLHMQTS SPTAAS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2 domains	[U-13C; U-15N]	protein	0.86 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2EE8, Strand ID: A Detail

---

Release date

2008-12-09

Citation

Solution structure of three zf-C2H2 domains from mouse protein odd-skipped-related 2 splicing isoform 2
Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.
Not known

Related entities 1. zf-C2H2 domains, : 1 : 9 : 257 entities Detail

Interaction partners 1. zf-C2H2 domains, : 3 interactors Detail

Experiments performed 2 experiments Detail