BMRB: 10149

Solution structure of the CH domain from human MICAL-2

Authors

Tomizawa, T., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Protein MICAL-2

Entity

1. Protein MICAL-2 (polymer, Thiol state: all free), 121 monomers, 13568.10 Da Detail

GSSGSSGDIR PSKLLTWCQQ QTEGYQHVNV TDLTTSWRSG LALCAIIHRF RPELINFDSL NEDDAVENNQ LAFDVAEREF GIPPVTTGKE MASAQEPDKL SMVMYLSKFY ELFRGTPLRP V

Formula weight

13568.1 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.5 %, Completeness: 97.4 %, Completeness (bb): 96.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.4 % (1362 of 1398)	97.5 % (708 of 726)	97.8 % (533 of 545)	95.3 % (121 of 127)
Backbone	96.2 % (685 of 712)	96.3 % (234 of 243)	96.6 % (343 of 355)	94.7 % (108 of 114)
Sidechain	98.5 % (787 of 799)	98.1 % (474 of 483)	99.0 % (300 of 303)	100.0 % (13 of 13)
Aromatic	100.0 % (116 of 116)	100.0 % (58 of 58)	100.0 % (56 of 56)	100.0 % (2 of 2)
Methyl	100.0 % (126 of 126)	100.0 % (63 of 63)	100.0 % (63 of 63)

1. CH domain

GSSGSSGDIR PSKLLTWCQQ QTEGYQHVNV TDLTTSWRSG LALCAIIHRF RPELINFDSL NEDDAVENNQ LAFDVAEREF GIPPVTTGKE MASAQEPDKL SMVMYLSKFY ELFRGTPLRP V

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	CH domain	[U-13C; U-15N]	protein	1.20 mM
2	d-Tris-HCl(pH 7.0)		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.06 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2E9K, Strand ID: A Detail

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Release date

2008-12-10

Citation

Solution structure of the CH domain from human MICAL-2
Tomizawa, T., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Protein MICAL-2, : 1 : 2 : 255 entities Detail

Interaction partners 1. Protein MICAL-2, : 8 interactors Detail

Experiments performed 2 experiments Detail