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solution structure of conotoxin pu14a
Authors
Zhang, B., Huang, F., Du, W.
Assembly
conotoxin pu14a
Entity
1. conotoxin pu14a (polymer, Thiol state: all disulfide bound), 19 monomers, 2065.524 Da Detail

DCPPHPVPGM HKCVCLKTC


Formula weight
2065.524 Da
Entity Connection
disulfide 2 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS2:SG1:CYS15:SG
2disulfidesing1:CYS13:SG1:CYS19:SG

Source organism
Conus pulicarius
Exptl. method
SOLUTION NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 96.4 %, Completeness (bb): 91.2 % Detail

Polymer type: polypeptide(L)

Total1H
All96.4 % (107 of 111)96.4 % (107 of 111)
Backbone91.2 % (31 of 34)91.2 % (31 of 34)
Sidechain98.7 % (76 of 77)98.7 % (76 of 77)
Aromatic100.0 % (4 of 4)100.0 % (4 of 4)
Methyl85.7 % (6 of 7)85.7 % (6 of 7)

1. conotoxin pu14a

DCPPHPVPGM HKCVCLKTC

Sample #1

Solvent system 90% H2O/10% D2O, Temperature 283 K, pH 3.0


#NameIsotope labelingTypeConcentration
1conotoxin pu14anatural abundanceprotein4 mM
2TFAnatural abundance0.01 %
3H2Onatural abundancesolvent90 %
4D2Onatural abundancesolvent10 %
Sample #2

Solvent system 100% D2O, Temperature 283 K, pH 3.0


#NameIsotope labelingTypeConcentration
5conotoxin pu14anatural abundanceprotein4 mM
6TFAnatural abundance0.01 %
7D2Onatural abundancesolvent100 %

Release date
2011-11-06
Citation
Solution structure of a novel α-conotoxin with a distinctive loop spacing pattern
Zhang, B., Huang, F., Du, W.
Amino Acids (2012), 43, 389-396, PubMed 21968500 , DOI 10.1007/s00726-011-1093-x ,
Related entities 1. conotoxin pu14a, : 1 : 1 entities Detail
Experiments performed 4 experiments Detail
Keywords pu14a