BMRB: 10159

Solution structure of the 16th filamin domain from human Filamin-B

Authors

Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Filamin-B

Entity

1. Filamin-B (polymer, Thiol state: all free), 95 monomers, 10168.31 Da Detail

GSSGSSGVSD MNGLGFKPFD LVIPFAVRKG EITGEVHMPS GKTATPEIVD NKDGTVTVRY APTEVGLHEM HIKYMGSHIP ESPLQFYVNY PNSGS

Formula weight

10168.31 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.3 %, Completeness (bb): 97.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.3 % (1027 of 1055)	96.9 % (528 of 545)	98.1 % (410 of 418)	96.7 % (89 of 92)
Backbone	97.3 % (539 of 554)	96.9 % (188 of 194)	97.8 % (267 of 273)	96.6 % (84 of 87)
Sidechain	97.6 % (570 of 584)	96.9 % (340 of 351)	98.7 % (225 of 228)	100.0 % (5 of 5)
Aromatic	95.5 % (84 of 88)	95.5 % (42 of 44)	95.5 % (42 of 44)
Methyl	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)

1. filamin domain

GSSGSSGVSD MNGLGFKPFD LVIPFAVRKG EITGEVHMPS GKTATPEIVD NKDGTVTVRY APTEVGLHEM HIKYMGSHIP ESPLQFYVNY PNSGS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	filamin domain	[U-13C; U-15N]	protein	1.21 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.52 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EE9, Strand ID: A Detail

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Release date

2009-02-19

Citation

Solution structure of the 16th filamin domain from human Filamin-B
Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Filamin-B, : 1 : 5 : 35 entities Detail

Interaction partners 1. Filamin-B, : 67 interactors Detail

Experiments performed 2 experiments Detail