BMRB: 10162

Solution structure of the 23th filamin domain from human Filamin-B

Authors

Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Filamin-B

Entity

1. Filamin-B (polymer, Thiol state: all free), 125 monomers, 12758.17 Da Detail

GSSGSSGFKV RVGEPGQAGN PALVSAYGTG LEGGTTGIQS EFFINTTRAG PGTLSVTIEG PSKVKMDCQE TPEGYKVMYT PMAPGNYLIS VKYGGPNHIV GSPFKAKVTG QRLVSPGSAN ETSSI

Formula weight

12758.17 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.2 %, Completeness: 88.0 %, Completeness (bb): 84.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.0 % (1180 of 1341)	89.3 % (619 of 693)	87.4 % (458 of 524)	83.1 % (103 of 124)
Backbone	84.9 % (620 of 730)	87.4 % (228 of 261)	84.2 % (298 of 354)	81.7 % (94 of 115)
Sidechain	91.6 % (655 of 715)	90.5 % (391 of 432)	93.1 % (255 of 274)	100.0 % (9 of 9)
Aromatic	88.1 % (74 of 84)	88.1 % (37 of 42)	88.1 % (37 of 42)
Methyl	93.3 % (112 of 120)	93.3 % (56 of 60)	93.3 % (56 of 60)

1. filamin domain, Filamin 23

GSSGSSGFKV RVGEPGQAGN PALVSAYGTG LEGGTTGIQS EFFINTTRAG PGTLSVTIEG PSKVKMDCQE TPEGYKVMYT PMAPGNYLIS VKYGGPNHIV GSPFKAKVTG QRLVSPGSAN ETSSI

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	filamin domain	[U-13C; U-15N]	protein	1.14 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	D2O		solvent	10 %
7	H2O		solvent	90 %

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LACS Plot; CA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.35 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EEC, Strand ID: A Detail

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Release date

2009-02-19

Citation

Solution structure of the 23th filamin domain from human Filamin-B
Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Filamin-B, : 1 : 3 : 166 entities Detail

Interaction partners 1. Filamin-B, : 68 interactors Detail

Experiments performed 2 experiments Detail