BMRB: 10195

Solution structure of the second SH3 domain of human Vinexin

Authors

Abe, H., Tochio, N., Miyamoto, K., Saito, K., Sasagawa, A., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Vinexin

Entity

1. Vinexin (polymer, Thiol state: all free), 90 monomers, 9914.901 Da Detail

GSSGSSGKPP TYQVLEYGEA VAQYTFKGDL EVELSFRKGE HICLIRKVNE NWYEGRITGT GRQGIFPASY VQVSREPRLR LCDDSGPSSG

Formula weight

9914.901 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 90.4 %, Completeness (bb): 87.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.4 % (922 of 1020)	91.8 % (490 of 534)	89.3 % (352 of 394)	87.0 % (80 of 92)
Backbone	87.5 % (464 of 530)	90.4 % (169 of 187)	86.0 % (222 of 258)	85.9 % (73 of 85)
Sidechain	93.5 % (531 of 568)	92.5 % (321 of 347)	94.9 % (203 of 214)	100.0 % (7 of 7)
Aromatic	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (42 of 42)	100.0 % (1 of 1)
Methyl	97.4 % (76 of 78)	97.4 % (38 of 39)	97.4 % (38 of 39)

1. SH3 domain

GSSGSSGKPP TYQVLEYGEA VAQYTFKGDL EVELSFRKGE HICLIRKVNE NWYEGRITGT GRQGIFPASY VQVSREPRLR LCDDSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1.00 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.29 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.29 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.39 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YUP, Strand ID: A Detail

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Release date

2009-02-26

Citation

Solution structure of the second SH3 domain of human Vinexin
Abe, H., Tochio, N., Miyamoto, K., Saito, K., Sasagawa, A., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Vinexin, : 1 : 2 : 44 entities Detail

Interaction partners 1. Vinexin, : 62 interactors Detail

Experiments performed 2 experiments Detail