BMRB: 10215

Solution structure of the short-isoform of the second WW domain from the human membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 (MAGI-1)

Authors

Ohnishi, S., Tochio, N., Tomizawa, T., Akasaka, R., Harada, T., Seki, E., Sato, M., Watanabe, S., Fujikura, Y., Koshiba, S., Terada, T., Shirouzu, M., Tanaka, A., Kigawa, T., Yokoyama, S.

Assembly

Membrane-associated guanylate kinase

Entity

1. Membrane-associated guanylate kinase (polymer, Thiol state: not present), 49 monomers, 5287.586 Da Detail

GSSGSSGLDS ELELPAGWEK IEDPVYGIYY VDHINRKTQY ENPSGPSSG

Formula weight

5287.586 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.9 %, Completeness: 92.7 %, Completeness (bb): 91.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.7 % (497 of 536)	91.8 % (256 of 279)	94.7 % (197 of 208)	89.8 % (44 of 49)
Backbone	91.3 % (261 of 286)	90.1 % (91 of 101)	92.9 % (130 of 140)	88.9 % (40 of 45)
Sidechain	94.2 % (275 of 292)	92.7 % (165 of 178)	96.4 % (106 of 110)	100.0 % (4 of 4)
Aromatic	100.0 % (48 of 48)	100.0 % (24 of 24)	100.0 % (23 of 23)	100.0 % (1 of 1)
Methyl	100.0 % (36 of 36)	100.0 % (18 of 18)	100.0 % (18 of 18)

1. WW domain1

GSSGSSGLDS ELELPAGWEK IEDPVYGIYY VDHINRKTQY ENPSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	WW domain1	[U-13C; U-15N]	protein	0.7 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -2.28 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -2.28 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -2.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2ZAJ, Strand ID: A Detail

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Release date

2009-04-03

Citation

Structural basis for controlling the dimerization and stability of the WW domains of an atypical subfamily
Ohnishi, S., Tochio, N., Tomizawa, T., Akasaka, R., Harada, T., Seki, E., Sato, M., Watanabe, S., Fujikura, Y., Koshiba, S., Terada, T., Shirouzu, M., Tanaka, A., Kigawa, T., Yokoyama, S.
Protein Sci. (2008), 17, 1531-1541, PubMed 18562638 , DOI 10.1110/ps.035329.108 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Membrane-associated guanylate kinase, : 1 : 119 entities Detail

Experiments performed 2 experiments Detail