Instrument
Bruker AVANCE DMX - 600 MHz + XYZ gradients, selective probe
Sample
State isotropic, Solvent system DMSO, Temperature 298 (±0.1) K, Details The peptide was dissolved in triple-distilled water (TDW) at pH 3.0. After
lyophilization the apo peptide (2.0 mg, 2.01 umol) was dissolved in DMSO-d6
(500 uL) and was used as a control solution. In a separate flask, the peptide
(2.0 mg, 2.01 umol) was dissolved in DMSO-d6 (450 uL) and CuCl was added from
a stock solution (50 uL, 40.0 mM) in DMSO-d6, giving a dilution factor of 10
and a final CuCl concentration of 4.0 mM. All work was performed in a nitrogen
glove-box. Samples remained colorless throughout the measurement.
| # | Name | Isotope labeling | Type | Concentration |
|---|
| 1 | MDCSGCSRPG | natural abundance | protein | 4.0 mM |
|---|
| 2 | DMSO | [U-2H] | | solvent mM |
|---|
| 3 | CuCl | natural abundance | | 4.0 mM |
|---|
