BMRB: 10235

Solution Structure of the C-terminal Phosphotyrosine Interaction Domain of APBB2 from Mouse

Authors

Li, H., Hayashi, F., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

amyloid beta (A4) precursor protein-bindin, family B, member 2

Entity

1. amyloid beta (A4) precursor protein-bindin, family B, member 2 (polymer, Thiol state: all free), 136 monomers, 14738.43 Da Detail

GSSGSSGPTP KTELVQKFRV QYLGMLPVDR PVGMDTLNSA IENLMTSSSK EDWPSVNMNV ADATVTVISE KNEEEVLVEC RVRFLSFMGV GKDVHTFAFI MDTGNQRFEC HVFWCEPNAA NVSEAVQAAC SGPSSG

Formula weight

14738.43 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 96.5 %, Completeness (bb): 95.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.5 % (1452 of 1504)	96.1 % (744 of 774)	96.9 % (569 of 587)	97.2 % (139 of 143)
Backbone	95.3 % (764 of 802)	94.2 % (259 of 275)	95.5 % (380 of 398)	96.9 % (125 of 129)
Sidechain	97.9 % (811 of 828)	97.2 % (485 of 499)	99.0 % (312 of 315)	100.0 % (14 of 14)
Aromatic	100.0 % (110 of 110)	100.0 % (55 of 55)	100.0 % (53 of 53)	100.0 % (2 of 2)
Methyl	99.3 % (137 of 138)	98.6 % (68 of 69)	100.0 % (69 of 69)

1. Phosphotyrosine-Interaction Domain

GSSGSSGPTP KTELVQKFRV QYLGMLPVDR PVGMDTLNSA IENLMTSSSK EDWPSVNMNV ADATVTVISE KNEEEVLVEC RVRFLSFMGV GKDVHTFAFI MDTGNQRFEC HVFWCEPNAA NVSEAVQAAC SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Phosphotyrosine-Interaction Domain	[U-13C; U-15N]	protein	0.84 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	2 mM
5	NaN3		salt	0.02 %
6	D2O		solvent	10 %
7	H2O		solvent	90 %

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.23 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WGU, Strand ID: A Detail

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Release date

2009-03-18

Citation

Structure of the C-terminal phosphotyrosine interaction domain of Fe65L1 complexed with the cytoplasmic tail of amyloid precursor protein reveals a novel peptide binding mode
Li, H., Koshiba, S., Hayashi, F., Tochio, N., Tomozawa, T., Kasai, T., Yabuki, T., Motoda, Y., Harada, T., Watanabe, S., Inoue, M., Hayashizaki, Y., Tanaka, A., Kigawa, T., Yokoyama, S.
J. Biol. Chem. (2008), 283, 27165-27178, PubMed 18650440 , DOI 10.1074/jbc.M803892200 , Abstract

Related entities 1. amyloid beta (A4) precursor protein-bindin, family B, member 2, : 1 : 2 : 4 : 59 entities Detail

Interaction partners 1. amyloid beta (A4) precursor protein-bindin, family B, member 2, : 1 interactors Detail

Experiments performed 2 experiments Detail