BMRB: 10239

Solution structure of the chimera of the C-terminal tail peptide of APP and the C-terminal PID domain of Fe65L

Authors

Li, H., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Amyloid beta A4 protein and Amyloid beta A4 precursor protein-binding family B member 2

Entity

1. Amyloid beta A4 protein and Amyloid beta A4 precursor protein-binding family B member 2 (polymer, Thiol state: all free), 185 monomers, 19989.95 Da Detail

GSSGSSGDAA VTPEERHLSK MQQNGYENPT YKFFEQMQNS GPSSGIEGRG SSGSSGSSGS SGPTPKTELV QKFRVQYLGM LPVDRPVGMD TLNSAIENLM TSSSKEDWPS VNMNVADATV TVISEKNEEE VLVECRVRFL SFMGVGKDVH TFAFIMDTGN QRFECHVFWC EPNAANVSEA VQAAC

Formula weight

19989.95 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.1 %, Completeness: 94.1 %, Completeness (bb): 92.1 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.1 % (1914 of 2035)	94.2 % (993 of 1054)	94.1 % (738 of 784)	92.9 % (183 of 197)
Backbone	92.1 % (1006 of 1092)	91.5 % (346 of 378)	92.6 % (498 of 538)	92.0 % (162 of 176)
Sidechain	96.0 % (1067 of 1111)	95.7 % (647 of 676)	96.4 % (399 of 414)	100.0 % (21 of 21)
Aromatic	95.3 % (143 of 150)	97.3 % (73 of 75)	93.2 % (68 of 73)	100.0 % (2 of 2)
Methyl	100.0 % (158 of 158)	100.0 % (79 of 79)	100.0 % (79 of 79)

1. APP peptide and PID domain

GSSGSSGDAA VTPEERHLSK MQQNGYENPT YKFFEQMQNS GPSSGIEGRG SSGSSGSSGS SGPTPKTELV QKFRVQYLGM LPVDRPVGMD TLNSAIENLM TSSSKEDWPS VNMNVADATV TVISEKNEEE VLVECRVRFL SFMGVGKDVH TFAFIMDTGN QRFECHVFWC EPNAANVSEA VQAAC

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	APP peptide and PID domain	[U-13C; U-15N]	protein	1.00 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2YT1, Strand ID: A Detail

---

Release date

2009-03-18

Citation

Structure of the C-terminal phosphotyrosine interaction domain of Fe65L1 complexed with the cytoplasmic tail of amyloid precursor protein reveals a novel peptide binding mode
Li, H., Koshiba, S., Hayashi, F., Tochio, N., Tomozawa, T., Kasai, T., Yabuki, T., Motoda, Y., Harada, T., Watanabe, S., Inoue, M., Hayashizaki, Y., Tanaka, A., Kigawa, T., Yokoyama, S.
J. Biol. Chem. (2008), 283, 27165-27178, PubMed 18650440 , DOI 10.1074/jbc.M803892200 , Abstract

Related entities 1. Amyloid beta A4 protein and Amyloid beta A4 precursor protein-binding family B member 2, : 1 : 4 entities Detail

Experiments performed 2 experiments Detail