BMRB: 21025

MDCSGCSRPG+Zn from acidic conditions

Authors

Shoshan, M.S., Shalev, D.E., Tshuva, E.Y.

Assembly

MDCSGCSRPG + Zn acidic

Entity

1. MDCSGCSRPG + Zn acidic (polymer, Thiol state: all free), 10 monomers, 1012.144 Da Detail

MDCSGCSRPG

Formula weight

1012.144 Da

Entity Connection

peptide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	peptide	sing	1:MET1:N	1:GLY10:C

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, conformer_family_coord_set

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.0 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	98.0 % (48 of 49)	98.0 % (48 of 49)
Backbone	100.0 % (21 of 21)	100.0 % (21 of 21)
Sidechain	96.4 % (27 of 28)	96.4 % (27 of 28)

1. MDCSGCSRPG+Zn

MDCSGCSRPG

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Sample

Solvent system 10% TDW/d6-DMSO, Temperature 298 (±0.1) K, Details The lyophilized peptide (2.0 mg, 2.01 umol) was dissolved in DMSO-d6 (450 uL) and a stock solution of ZnCl2 (50 uL, 40.0 mM) in TDW was added, giving a dilution factor of 10 and a final concentration of 4.0 mM.

#	Name	Isotope labeling	Type	Concentration
1	MDCSGCSRPG	natural abundance	protein	4.0 mM
2	ZnCl2	natural abundance		4.0 mM
3	DMSO	[U-2H]		90 %
4	H2O	natural abundance		10 %

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Release date

2013-03-21

Citation

Peptide models of Cu(I) and Zn(II) metallochaperones: the effect of pH on coordination and mechanistic implications
Shoshan, M.S., Shalev, D.E., Tshuva, E.Y.
Inorg. Chem. (2013), 52, 2993-3000, PubMed 23458158 , DOI 10.1021/ic302404w , Abstract

Entries sharing articles BMRB: 6 entries Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail