BMRB: 10264

The solution structure of the VHS domain of human Signal transducing adaptor molecule 2

Authors

Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

signal transducing adaptor molecule 2

Entity

1. signal transducing adaptor molecule 2 (polymer, Thiol state: all free), 163 monomers, 17663.02 Da Detail

GSSGSSGMPL FTANPFEQDV EKATNEYNTT EDWSLIMDIC DKVGSTPNGA KDCLKAIMKR VNHKVPHVAL QALTLLGACV ANCGKIFHLE VCSRDFATEV RAVIKNKAHP KVCEKLKSLM VEWSEEFQKD PQFSLISATI KSMKEEGITF PPAGSQTSGP SSG

Formula weight

17663.02 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 95.1 %, Completeness (bb): 96.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.1 % (1758 of 1848)	92.6 % (889 of 960)	98.2 % (707 of 720)	96.4 % (162 of 168)
Backbone	96.8 % (929 of 960)	95.4 % (313 of 328)	97.9 % (468 of 478)	96.1 % (148 of 154)
Sidechain	94.0 % (978 of 1040)	91.1 % (576 of 632)	98.5 % (388 of 394)	100.0 % (14 of 14)
Aromatic	100.0 % (118 of 118)	100.0 % (59 of 59)	100.0 % (57 of 57)	100.0 % (2 of 2)
Methyl	100.0 % (166 of 166)	100.0 % (83 of 83)	100.0 % (83 of 83)

1. VHS domain

GSSGSSGMPL FTANPFEQDV EKATNEYNTT EDWSLIMDIC DKVGSTPNGA KDCLKAIMKR VNHKVPHVAL QALTLLGACV ANCGKIFHLE VCSRDFATEV RAVIKNKAHP KVCEKLKSLM VEWSEEFQKD PQFSLISATI KSMKEEGITF PPAGSQTSGP SSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	VHS domain	[U-13C; U-15N]	protein	1.5 mM
2	d-Tris HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	5 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.3 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.3 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.54 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X5B, Strand ID: A Detail

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Release date

2010-03-10

Citation

The solution structure of the VHS domain of human Signal transducing adaptor molecule 2
Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. signal transducing adaptor molecule 2, : 1 : 1 : 2 : 182 entities Detail

Interaction partners 1. signal transducing adaptor molecule 2, : 35 interactors Detail

Experiments performed 2 experiments Detail