BMRB: 10298

structure of the third Homeodomain from the human homeobox and leucine zipper protein, Homez

Authors

Ohnishi, S., Tomizawa, T., Koshiba, S., Harada, T., Watanabe, S., Kigawa, T., Yokoyama, S.

Assembly

Homeobox and leucine zipper protein Homez

Entity

1. Homeobox and leucine zipper protein Homez (polymer, Thiol state: not present), 70 monomers, 7560.233 Da Detail

GSSGSSGPLP IPPPPPDIQP LERYWAAHQQ LRETDIPQLS QASRLSTQQV LDWFDSRLPQ PAEVSGPSSG

Formula weight

7560.233 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.6 %, Completeness: 87.3 %, Completeness (bb): 87.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	87.3 % (692 of 793)	85.0 % (357 of 420)	89.5 % (273 of 305)	91.2 % (62 of 68)
Backbone	87.1 % (345 of 396)	85.0 % (113 of 133)	87.8 % (180 of 205)	89.7 % (52 of 58)
Sidechain	87.7 % (405 of 462)	85.0 % (244 of 287)	91.5 % (151 of 165)	100.0 % (10 of 10)
Aromatic	100.0 % (46 of 46)	100.0 % (23 of 23)	100.0 % (21 of 21)	100.0 % (2 of 2)
Methyl	100.0 % (60 of 60)	100.0 % (30 of 30)	100.0 % (30 of 30)

1. homeodomain

GSSGSSGPLP IPPPPPDIQP LERYWAAHQQ LRETDIPQLS QASRLSTQQV LDWFDSRLPQ PAEVSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	homeodomain	[U-13C; U-15N]	protein	1.0 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.3 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YS9, Strand ID: A Detail

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Release date

2009-12-16

Citation

structure of the third Homeodomain from the human homeobox and leucine zipper protein, Homez
Ohnishi, S., Tomizawa, T., Koshiba, S., Harada, T., Watanabe, S., Kigawa, T., Yokoyama, S.

Related entities 1. Homeobox and leucine zipper protein Homez, : 1 : 2 : 8 entities Detail

Interaction partners 1. Homeobox and leucine zipper protein Homez, : 35 interactors Detail

Experiments performed 2 experiments Detail