BMRB: 10320

Solution structure of the 9th filamin domain from human Filamin-B

Authors

Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Filamin-B

Entity

1. Filamin-B (polymer, Thiol state: all free), 131 monomers, 13196.37 Da Detail

GSSGSSGDVT YDGHPVPGSP YTVEASLPPD PSKVKAHGPG LEGGLVGKPA EFTIDTKGAG TGGLGLTVEG PCEAKIECSD NGDGTCSVSY LPTKPGEYFV NILFEEVHIP GSPFKADIEM PFDPSSGPSS G

Formula weight

13196.37 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.7 %, Completeness: 94.4 %, Completeness (bb): 92.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.4 % (1290 of 1367)	94.5 % (666 of 705)	94.7 % (516 of 545)	92.3 % (108 of 117)
Backbone	92.4 % (697 of 754)	92.2 % (247 of 268)	92.7 % (344 of 371)	92.2 % (106 of 115)
Sidechain	96.4 % (696 of 722)	95.9 % (419 of 437)	97.2 % (275 of 283)	100.0 % (2 of 2)
Aromatic	95.7 % (90 of 94)	95.7 % (45 of 47)	95.7 % (45 of 47)
Methyl	100.0 % (112 of 112)	100.0 % (56 of 56)	100.0 % (56 of 56)

1. Filamin domain

GSSGSSGDVT YDGHPVPGSP YTVEASLPPD PSKVKAHGPG LEGGLVGKPA EFTIDTKGAG TGGLGLTVEG PCEAKIECSD NGDGTCSVSY LPTKPGEYFV NILFEEVHIP GSPFKADIEM PFDPSSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Filamin domain	[U-13C; U-15N]	protein	1.11 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.1 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.1 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.61 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DI9, Strand ID: A Detail

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Release date

2010-04-06

Citation

Solution structure of the 9th filamin domain from human Filamin-B
Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Filamin-B, : 1 : 381 entities Detail

Interaction partners 1. Filamin-B, : 62 interactors Detail

Experiments performed 2 experiments Detail