BMRB: 10323

Solution structure of the 13th filamin domain from human Filamin-B

Authors

Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Filamin-B

Entity

1. Filamin-B (polymer, Thiol state: not present), 108 monomers, 11185.47 Da Detail

GSSGSSGVVD PSKVKIAGPG LGSGVRARVL QSFTVDSSKA GLAPLEVRVL GPRGLVEPVN VVDNGDGTHT VTYTPSQEGP YMVSVKYADE EIPRSPFKVK VLPTYDAS

Formula weight

11185.47 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 94.5 %, Completeness (bb): 93.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.5 % (1102 of 1166)	94.0 % (565 of 601)	95.7 % (443 of 463)	92.2 % (94 of 102)
Backbone	93.0 % (584 of 628)	91.8 % (201 of 219)	94.2 % (293 of 311)	91.8 % (90 of 98)
Sidechain	96.2 % (609 of 633)	95.3 % (364 of 382)	97.6 % (241 of 247)	100.0 % (4 of 4)
Aromatic	100.0 % (56 of 56)	100.0 % (28 of 28)	100.0 % (28 of 28)
Methyl	95.3 % (122 of 128)	93.8 % (60 of 64)	96.9 % (62 of 64)

1. Filamin domain

GSSGSSGVVD PSKVKIAGPG LGSGVRARVL QSFTVDSSKA GLAPLEVRVL GPRGLVEPVN VVDNGDGTHT VTYTPSQEGP YMVSVKYADE EIPRSPFKVK VLPTYDAS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Filamin domain	[U-13C; U-15N]	protein	1.16 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.03 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.03 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.94 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DJ4, Strand ID: A Detail

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Release date

2010-04-06

Citation

Solution structure of the 13th filamin domain from human Filamin-B
Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Filamin-B, : 1 : 2 : 453 entities Detail

Interaction partners 1. Filamin-B, : 62 interactors Detail

Experiments performed 2 experiments Detail