BMRB: 10324

Solution structure of the 20th Filamin domain from human Filamin-B

Authors

Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Filamin-B

Entity

1. Filamin-B (polymer, Thiol state: all free), 102 monomers, 10368.46 Da Detail

GSSGSSGRAP SVATVGSICD LNLKIPEINS SDMSAHVTSP SGRVTEAEIV PMGKNSHCVR FVPQEMGVHT VSVKYRGQHV TGSPFQFTVG PLGEGGSGPS SG

Formula weight

10368.46 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.1 %, Completeness: 93.7 %, Completeness (bb): 91.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.7 % (997 of 1064)	93.1 % (510 of 548)	95.2 % (396 of 416)	91.0 % (91 of 100)
Backbone	91.6 % (546 of 596)	90.0 % (190 of 211)	93.1 % (271 of 291)	90.4 % (85 of 94)
Sidechain	96.2 % (534 of 555)	95.0 % (320 of 337)	98.1 % (208 of 212)	100.0 % (6 of 6)
Aromatic	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (27 of 27)
Methyl	97.9 % (94 of 96)	95.8 % (46 of 48)	100.0 % (48 of 48)

1. Filamin domain

GSSGSSGRAP SVATVGSICD LNLKIPEINS SDMSAHVTSP SGRVTEAEIV PMGKNSHCVR FVPQEMGVHT VSVKYRGQHV TGSPFQFTVG PLGEGGSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Filamin domain	[U-13C; U-15N]	protein	1.14 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 7 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.49 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DLG, Strand ID: A Detail

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Release date

2010-04-06

Citation

Solution structure of the 20th Filamin domain from human Filamin-B
Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Filamin-B, : 1 : 83 entities Detail

Interaction partners 1. Filamin-B, : 68 interactors Detail

Experiments performed 2 experiments Detail