BMRB: 10325

Solution structure of the 15th Filamin domain from human Filamin-B

Authors

Tomizawa, T., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Filamin-B

Entity

1. Filamin-B (polymer, Thiol state: all free), 124 monomers, 12345.40 Da Detail

GSSGSSGTGD ASKCLATGPG IASTVKTGEE VGFVVDAKTA GKGKVTCTVL TPDGTEAEAD VIENEDGTYD IFYTAAKPGT YVIYVRFGGV DIPNSPFTVM ATDGEVTAVE EAPVNACPSG PSSG

Formula weight

12345.4 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 81.5 %, Completeness: 81.3 %, Completeness (bb): 79.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	81.3 % (1015 of 1248)	80.4 % (505 of 628)	82.4 % (413 of 501)	81.5 % (97 of 119)
Backbone	79.3 % (577 of 728)	77.5 % (200 of 258)	79.7 % (282 of 354)	81.9 % (95 of 116)
Sidechain	83.4 % (522 of 626)	82.4 % (305 of 370)	85.0 % (215 of 253)	66.7 % (2 of 3)
Aromatic	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (36 of 36)
Methyl	83.6 % (117 of 140)	82.9 % (58 of 70)	84.3 % (59 of 70)

1. Filamin domain

GSSGSSGTGD ASKCLATGPG IASTVKTGEE VGFVVDAKTA GKGKVTCTVL TPDGTEAEAD VIENEDGTYD IFYTAAKPGT YVIYVRFGGV DIPNSPFTVM ATDGEVTAVE EAPVNACPSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Filamin domain	[U-13C; U-15N]	protein	1.21 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.21 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.21 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.46 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DMB, Strand ID: A Detail

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Release date

2010-04-06

Citation

Solution structure of the 15th Filamin domain from human Filamin-B
Tomizawa, T., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Filamin-B, : 1 : 51 entities Detail

Interaction partners 1. Filamin-B, : 66 interactors Detail

Experiments performed 2 experiments Detail