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Solution structure of the 15th Filamin domain from human Filamin-B
Authors
Tomizawa, T., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.
Assembly
Filamin-B
Entity
1. Filamin-B (polymer, Thiol state: all free), 124 monomers, 12345.40 Da Detail

GSSGSSGTGD ASKCLATGPG IASTVKTGEE VGFVVDAKTA GKGKVTCTVL TPDGTEAEAD VIENEDGTYD IFYTAAKPGT YVIYVRFGGV DIPNSPFTVM ATDGEVTAVE EAPVNACPSG PSSG


Formula weight
12345.4 Da
Source organism
Homo sapiens
Exptl. method
SOLUTION NMR
Refine. method
torsion angle dynamics, restrainted molecular dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 83.1 %, Completeness: 84.0 %, Completeness (bb): 82.5 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All84.0 % (1049 of 1249)83.3 % (523 of 628)85.2 % (427 of 501)82.5 % (99 of 120)
Backbone82.5 % (586 of 710)82.1 % (197 of 240)82.5 % (292 of 354)83.6 % (97 of 116)
Sidechain85.4 % (551 of 645)84.0 % (326 of 388)88.1 % (223 of 253)50.0 % (2 of 4)
Aromatic100.0 % (72 of 72)100.0 % (36 of 36)100.0 % (36 of 36)
Methyl88.6 % (124 of 140)88.6 % (62 of 70)88.6 % (62 of 70)

1. Filamin domain

GSSGSSGTGD ASKCLATGPG IASTVKTGEE VGFVVDAKTA GKGKVTCTVL TPDGTEAEAD VIENEDGTYD IFYTAAKPGT YVIYVRFGGV DIPNSPFTVM ATDGEVTAVE EAPVNACPSG PSSG

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)


#NameIsotope labelingTypeConcentration
1Filamin domain[U-13C; U-15N]protein1.21 mM
2d-Tris-HClnatural abundancebuffer20 mM
3NaClnatural abundancesalt100 mM
4d-DTTnatural abundancesalt1 mM
5NaN3natural abundancesalt0.02 %
6H2Osolvent90 %
7D2Osolvent10 %

LACS Plot; CA
Referencing offset: 0.21 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.21 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.12 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.46 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2DMB, Strand ID: A Detail


Release date
2010-04-06
Citation
Solution structure of the 15th Filamin domain from human Filamin-B
Tomizawa, T., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.
Related entities 1. Filamin-B, : 1 : 31 entities Detail
Interaction partners 1. Filamin-B, : 63 interactors Detail
Experiments performed 2 experiments Detail