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Found 1 Document (0.232 seconds) for PDB ID: 1062

BMRB: 21062

conotoxin Im10A

Authors

Jiang, L.

Assembly

conotoxin Im10A

Entity

1. conotoxin Im10A (polymer, Thiol state: all disulfide bound), 11 monomers, 1239.485 Da Detail

NTICCEGCMC Y

Formula weight

1239.485 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS4:SG	1:CYS10:SG
2	disulfide	sing	1:CYS5:SG	1:CYS8:SG

Source organism

Conus imperialis

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.9 %, Completeness: 87.7 %, Completeness (bb): 91.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	87.7 % (50 of 57)	87.7 % (50 of 57)
Backbone	91.3 % (21 of 23)	91.3 % (21 of 23)
Sidechain	85.3 % (29 of 34)	85.3 % (29 of 34)
Aromatic	100.0 % (4 of 4)	100.0 % (4 of 4)
Methyl	100.0 % (3 of 3)	100.0 % (3 of 3)

1. Im10A

NTICCEGCMC Y

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	entity	natural abundance	protein	0.3 ~ 0.5 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

Release date

2016-05-23

Citation

Im10A, a short conopeptide isolated from Conus imperialis and possesses two highly concentrated disulfide bridges and analgesic activity
Yua, S., Du, T., Liu, Z., Wu, Q., Feng, G., Dong, M., Zhou, X., Jiang, L., Dai, Q.
Peptides (2016), 81, 15-20, PubMed 27131596 , DOI 10.1016/j.peptides.2016.04.004 , Abstract

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr21062_3.str
Input source #2: Coordindates	bmr21062.coor.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	True (see coordinates for details)
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:4:CYS:SG	1:10:CYS:SG	unknown	unknown	2.04
1:5:CYS:SG	1:8:CYS:SG	unknown	unknown	2.033

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): 1 vs Auth_asym_ID (model): A

--------10-
NTICCEGCMCY
|||||||||||
NTICCEGCMCY

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
1	A	11	0	0	100.0

Content subtype: bmr21062_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	56		90.9 (chain: 1, length: 11)
1	Chemical shift references	chemical_shift_reference_1	OK	1		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 11, Sequence coverage: 90.9%
- Total number of assignments
  - ¹H chemical shifts: 56
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	57	50	87.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	23	21	91.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	34	29	85.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	4	3	75.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	4	4	100.0

Keywords conotoxin Im10A

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Found 1 Document (0.232 seconds) for PDB ID: 1062

BMRB: 21062

Sample

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail