BMRBj - BMREntryMaster

Found 1 Document (6.919 seconds)

BMRB: 11032

2RO0

Solution structure of the knotted tudor domain of the yeast histone acetyltransferase protein, Esa1

Authors

Shimojo, H., Sano, N., Moriwaki, Y., Okuda, M., Horikoshi, M., Nishimura, Y.

Assembly

the knotted tudor domain

Entity

1. the knotted tudor domain (polymer, Thiol state: all free), 92 monomers, 10773.08 Da Detail

GSHMSHDGKE EPGIAKKINS VDDIIIKCQC WVQKNDEERL AEILSINTRK APPKFYVHYV NYNKRLDEWI TTDRINLDKE VLYPKLKATD ED

Formula weight

10773.08 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.7 %, Completeness (bb): 95.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.7 % (1078 of 1115)	96.9 % (569 of 587)	97.0 % (417 of 430)	93.9 % (92 of 98)
Backbone	95.4 % (519 of 544)	95.6 % (175 of 183)	95.6 % (261 of 273)	94.3 % (83 of 88)
Sidechain	97.7 % (645 of 660)	97.5 % (394 of 404)	98.4 % (242 of 246)	90.0 % (9 of 10)
Aromatic	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (37 of 37)	100.0 % (2 of 2)
Methyl	100.0 % (96 of 96)	100.0 % (48 of 48)	100.0 % (48 of 48)

1. Residues 1-89

GSHMSHDGKE EPGIAKKINS VDDIIIKCQC WVQKNDEERL AEILSINTRK APPKFYVHYV NYNKRLDEWI TTDRINLDKE VLYPKLKATD ED

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Residues 1-89	[U-99% 13C; U-99% 15N]	0.35 mM
2	potassium phosphate		200 mM
3	H2O		95 %
4	D2O		5 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	Residues 1-89	natural abundance	0.35 mM
6	potassium phosphate		200 mM
7	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.37 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RO0, Strand ID: A Detail

Release date

2008-04-15

Citation

Novel structural and functional mode of a knot essential for RNA binding activity of the Esa1 presumed chromodomain
Shimojo, H., Sano, N., Moriwaki, Y., Okuda, M., Horikoshi, M., Nishimura, Y.
J. Mol. Biol. (2008), 378, 987-1001, PubMed 18407291 , DOI 10.1016/j.jmb.2008.03.021 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 11033 released on 2008-04-15
Title Solution structure of the presumed chromodomain of the yeast histone acetyltransferase protein, Esa1

Related entities 1. the knotted tudor domain, : 1 : 2 : 12 entities Detail

Interaction partners 1. the knotted tudor domain, : 42 interactors Detail

More details for 42 interactors identified by 10 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Residues 1-89	[U-99% 13C; U-99% 15N]	0.35 mM
2	potassium phosphate		200 mM
3	H2O		95 %
4	D2O		5 %

2 3D CBCA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Residues 1-89	[U-99% 13C; U-99% 15N]	0.35 mM
2	potassium phosphate		200 mM
3	H2O		95 %
4	D2O		5 %

3 3D HNCACB

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Residues 1-89	[U-99% 13C; U-99% 15N]	0.35 mM
2	potassium phosphate		200 mM
3	H2O		95 %
4	D2O		5 %

4 3D HNCO

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Residues 1-89	[U-99% 13C; U-99% 15N]	0.35 mM
2	potassium phosphate		200 mM
3	H2O		95 %
4	D2O		5 %

5 3D HNCA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Residues 1-89	[U-99% 13C; U-99% 15N]	0.35 mM
2	potassium phosphate		200 mM
3	H2O		95 %
4	D2O		5 %

6 3D HN(CO)CA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Residues 1-89	[U-99% 13C; U-99% 15N]	0.35 mM
2	potassium phosphate		200 mM
3	H2O		95 %
4	D2O		5 %

7 2D 1H-13C HSQC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	Residues 1-89	natural abundance	0.35 mM
6	potassium phosphate		200 mM
7	D2O		100 %

8 3D HCCH-TOCSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	Residues 1-89	natural abundance	0.35 mM
6	potassium phosphate		200 mM
7	D2O		100 %

9 3D 1H-15N NOESY

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Residues 1-89	[U-99% 13C; U-99% 15N]	0.35 mM
2	potassium phosphate		200 mM
3	H2O		95 %
4	D2O		5 %

10 3D 1H-13C NOESY

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	Residues 1-89	natural abundance	0.35 mM
6	potassium phosphate		200 mM
7	D2O		100 %

nullKeywords chromodomain, Esa1, HAT, RNA binding, tudor domain

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (6.919 seconds)

BMRB: 11032

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. the knotted tudor domain, : 1 : 2 : 12 entities Detail

Interaction partners 1. the knotted tudor domain, : 42 interactors Detail

Experiments performed 10 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample