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Found 1 Document (0.913 seconds)

BMRB: 11033

2RNZ

Solution structure of the presumed chromodomain of the yeast histone acetyltransferase protein, Esa1

Authors

Shimojo, H., Sano, N., Moriwaki, Y., Okuda, M., Horikoshi, M., Nishimura, Y.

Assembly

the presumed chromodomain

Entity

1. the presumed chromodomain (polymer, Thiol state: all free), 94 monomers, 11050.35 Da Detail

MGSSHHHHHH SSGLVPRGSH MSVDDIIIKC QCWVQKNDEE RLAEILSINT RKAPPKFYVH YVNYNKRLDE WITTDRINLD KEVLYPKLKA TDED

Formula weight

11050.35 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.9 %, Completeness (bb): 96.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.9 % (1045 of 1125)	92.7 % (545 of 588)	92.7 % (406 of 438)	94.9 % (94 of 99)
Backbone	96.4 % (536 of 556)	96.3 % (180 of 187)	96.8 % (270 of 279)	95.6 % (86 of 90)
Sidechain	90.5 % (597 of 660)	91.0 % (365 of 401)	89.6 % (224 of 250)	88.9 % (8 of 9)
Aromatic	60.2 % (59 of 98)	59.2 % (29 of 49)	59.6 % (28 of 47)	100.0 % (2 of 2)
Methyl	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (47 of 47)

1. Residues 17-89

MGSSHHHHHH SSGLVPRGSH MSVDDIIIKC QCWVQKNDEE RLAEILSINT RKAPPKFYVH YVNYNKRLDE WITTDRINLD KEVLYPKLKA TDED

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Residues 17-89	[U-99% 13C; U-99% 15N]	0.1 mM
2	potassium phosphate		200 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	Residues 17-89	[U-99% 13C; U-99% 15N]	0.1 mM
6	potassium phosphate		200 mM
7	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.48 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RNZ, Strand ID: A Detail

Release date

2008-04-15

Citation

Novel structural and functional mode of a knot essential for RNA binding activity of the Esa1 presumed chromodomain
Shimojo, H., Sano, N., Moriwaki, Y., Okuda, M., Horikoshi, M., Nishimura, Y.
J. Mol. Biol. (2008), 378, 987-1001, PubMed 18407291 , DOI 10.1016/j.jmb.2008.03.021 , Abstract

Related entities 1. the presumed chromodomain, : 1 : 14 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Residues 17-89	[U-99% 13C; U-99% 15N]	0.1 mM
2	potassium phosphate		200 mM
3	H2O		90 %
4	D2O		10 %

2 3D CBCA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Residues 17-89	[U-99% 13C; U-99% 15N]	0.1 mM
2	potassium phosphate		200 mM
3	H2O		90 %
4	D2O		10 %

3 3D HNCACB

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Residues 17-89	[U-99% 13C; U-99% 15N]	0.1 mM
2	potassium phosphate		200 mM
3	H2O		90 %
4	D2O		10 %

4 3D HNCO

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Residues 17-89	[U-99% 13C; U-99% 15N]	0.1 mM
2	potassium phosphate		200 mM
3	H2O		90 %
4	D2O		10 %

5 3D HNCA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Residues 17-89	[U-99% 13C; U-99% 15N]	0.1 mM
2	potassium phosphate		200 mM
3	H2O		90 %
4	D2O		10 %

6 3D HN(CO)CA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Residues 17-89	[U-99% 13C; U-99% 15N]	0.1 mM
2	potassium phosphate		200 mM
3	H2O		90 %
4	D2O		10 %

7 2D 1H-13C HSQC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	Residues 17-89	[U-99% 13C; U-99% 15N]	0.1 mM
6	potassium phosphate		200 mM
7	D2O		100 %

8 3D HCCH-TOCSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	Residues 17-89	[U-99% 13C; U-99% 15N]	0.1 mM
6	potassium phosphate		200 mM
7	D2O		100 %

9 3D 1H-15N NOESY

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Residues 17-89	[U-99% 13C; U-99% 15N]	0.1 mM
2	potassium phosphate		200 mM
3	H2O		90 %
4	D2O		10 %

10 3D 1H-13C NOESY

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	Residues 17-89	[U-99% 13C; U-99% 15N]	0.1 mM
6	potassium phosphate		200 mM
7	D2O		100 %

nullKeywords chromodomain, Esa1, HAT, RNA binding, tudor domain

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Found 1 Document (0.913 seconds)

BMRB: 11033

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. the presumed chromodomain, : 1 : 14 entities Detail

Experiments performed 10 experiments Detail

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