BMRB: 11052

Two-disulfide variant of hen lysozyme: 2SS[6-127, 64-80]

Authors

Sakamoto, K., Hirai, K., Kitamura, Y., Yamazaki, K., Yusa, M., Tokunaga, N., Doi, G., Noda, Y., Tachibana, H., Segawa, S.

Assembly

2SS(6-127 64-80)

Entity

1. 2SS(6-127 64-80) (polymer, Thiol state: all disulfide bound), 130 monomers, 14315.94 Da Detail

MKVFGRCELA AAMKRHGLDN YRGYSLGNWV AAAKFESNFN TQATNRNTDG STDYGILQIN SRWWCNDGRT PGSRNLANIP CSALLSSDIT ASVNAAKKIV SDGNGMNAWV AWRNRAKGTD VQAWIRGCRL

Formula weight

14315.94 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS128:SG
2	disulfide	sing	1:CYS65:SG	1:CYS81:SG

Source organism

Gallus gallus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.9 %, Completeness: 67.0 %, Completeness (bb): 92.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	67.0 % (607 of 906)	63.7 % (481 of 755)	83.4 % (126 of 151)
Backbone	92.5 % (368 of 398)	90.4 % (244 of 270)	96.9 % (124 of 128)
Sidechain	47.0 % (239 of 508)	48.9 % (237 of 485)	8.7 % (2 of 23)
Aromatic	0.0 % (0 of 71)	0.0 % (0 of 65)	0.0 % (0 of 6)
Methyl	60.3 % (38 of 63)	60.3 % (38 of 63)

1. 2SS(6-127 64-80)

MKVFGRCELA AAMKRHGLDN YRGYSLGNWV AAAKFESNFN TQATNRNTDG STDYGILQIN SRWWCNDGRT PGSRNLANIP CSALLSSDIT ASVNAAKKIV SDGNGMNAWV AWRNRAKGTD VQAWIRGCRL

Show sample condition

Sample #1

Solvent system 95% DMSO/5% H2O, Pressure 1 atm, Temperature 298 K, Details The pH was adjusted by adding DCl and NaOD.

#	Name	Isotope labeling	Type	Concentration
1	2SS(6-127_64-80)	[U-99% 15N]	protein	1 mM
2	dichloroacetic acid	[U-2H]		60 mM
3	DMSO-d6	[U-2H]	solvent	95 %
4	H2O		solvent	5 %

Sample #2

Solvent system 95% DMSO/5% H2O, Pressure 1 atm, Temperature 298 K, Details The pH was adjusted by adding DCl and NaOD.

#	Name	Isotope labeling	Type	Concentration
5	2SS(6-127_64-80)	[U-99% 15N]	protein	0.2 mM
6	dichloroacetic acid	[U-2H]		60 mM
7	DMSO-d6	[U-2H]	solvent	95 %
8	H2O		solvent	5 %

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Release date

2008-07-22

Citation

Glycerol-induced folding of unstructured disulfide-deficient lysozyme into a native-like conformation
Sakamoto, K., Hirai, K., Kitamura, Y., Yamazaki, K., Yusa, M., Tokunaga, N., Doi, G., Noda, Y., Tachibana, H., Segawa, S.
Biopolymers (2009), 91, 665-675, PubMed 19353641 , DOI 10.1002/bip.21198 , Abstract

Related entities 1. 2SS(6-127 64-80), : 1 : 105 : 141 entities Detail

Interaction partners 1. 2SS(6-127 64-80), : 7 interactors Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail