BMRB: 11074

Chemical shifts assignment for the segmentally labelled beta subunit (391-473 residues) of the F1-ATPase in the F1 complex

Authors

Yagi, H.

Assembly

the F1-ATPase in the F1 complex

Entity

1. F1 ATPase beta subunit (polymer, Thiol state: not present), 473 monomers, 51852.53 × 3 Da Detail

MTRGRVIQVM GPVVDVKFEN GHLPAIYNAL KIQHKARNEN EVDIDLTLEV ALHLGDDTVR TIAMASTDGL IRGMEVIDTG APISVPVGQV TLGRVFNVLG EPIDLEGDIP ADARRDPIHR PAPKFEELAT EVEILETGIK VVDLLAPYIK GGKIGLFGGA GVGKTVLIQE LIHNIAQEHG GISVFAGVGE RTREGNDLYH EMKDSGVISK TAMVFGQMNE PPGARMRVAL TGLTMAEYFR DEQGQDGLLF IDNIFRFTQA GSEVSALLGR MPSAIGYQPT LATEMGQLQE RITSTAKGSI TSIQAIYVPA DDYTDPAPAT TFSHLDATTN LERKLAEMGI YPAVDPLVST SRALAPEIVG EEHYQVARKV QQTLERYKEL QDIIAILGMD ELSDEDKLVV HRARRIQFFL SQNFHVAEQF TGQPGSYVPV KETVRGFKEI LEGKYDHLPE DAFRLVGRIE EVVEKAKAMG VEV

2. F1 ATPase alpha subunit (polymer, Thiol state: all free), 502 monomers, 54589.87 × 3 Da Detail

MSIRAEEISA LIKQQIENYE SQIQVSDVGT VIQVGDGIAR AHGLDNVMSG EAVEFANAVM GMALNLEENN VGIVILGPYT GIKEGDEVRR TGRIMEVPVG ETLIGRVVNP LGQPVDGLGP VETTETRPIE SRAPGVMDRR SVHEPLQTGI KAIDALVPIG RGQRELIIGD RQTGKTSVAI DTIINQKDQN MICIYVAIGQ KESTVATVVE TLAKHGAPDY TIVVTASASQ PAPLLFLAPY AGVAMGEYFM IMGKHVLVVI DDLSKQAAAY RQLSLLLRRP PGREAYPGDI FYLHSRLLER AAKLSDAKGG GSLTALPFVE TQAGDISAYI PTNVISITDG QIFLQSDLFF SGVRPAINAG LSVSRVGGAA QIKAMKKVAG TLRLDLAAYR ELEAFAQFGS DLDKATQANV ARGARTVEVL KQDLHQPIPV EKQVLIIYAL TRGFLDDIPV EDVRRFEKEF YLWLDQNGQH LLEHIRTTKD LPNEDDLNQA IEAFKKTFVV SQ

3. F1 ATPase gamma subunit (polymer, Thiol state: not present), 282 monomers, 31778.22 Da Detail

ASLRDIKTRI NATKKTSQIT KAMEMVLTSK LNRAEKREIV RPYMEKIQEV VANVALAARA SHPMLVSRPV KKTGYLVITS DRGLAGAYNS NVLRLVYQTI QKRHASPDEY AIIVIGRVGL SFFRKRNMPV ILDITRLPDQ PSFADIKEIA RKTVGLFADG TFDELYMYYN HYVSAIQQEV TERKLLPLTD LAENKQRTVY EFEPSQEEIL DVLLPQYAES LIYGALLDAK ASEHAARMTA MKNATDNANE LIRTLTLSYN RARQAAITQE ITEIVAGANA LQ

4. F1 ATPase epsilon subunit (polymer, Thiol state: not present), 90 monomers, 9522.858 Da Detail

MKTIHVSVVT PDGPVYEDDV EMVSVKAKSG ELGILPGHIP LVAPLEISAA RLKKGGKTQY IAVSGGFLEV RPDNVTILAQ AAERAEDIDV

Total weight

360628.25 Da

Max. entity weight

54589.87 Da

Source organism

Bacillus sp. PS3

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 8.3 %, Completeness: 3.0 %, Completeness (bb): 5.9 % Detail

Download

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Polymer type: polypeptide(L)

	Total	1H	15N
All	3.0 % (280 of 9362)	2.2 % (173 of 7972)	7.7 % (107 of 1390)
Backbone	5.9 % (236 of 4021)	4.7 % (129 of 2736)	8.3 % (107 of 1285)
Sidechain	0.8 % (45 of 5341)	0.9 % (45 of 5236)	0.0 % (0 of 105)
Aromatic	0.5 % (2 of 414)	0.5 % (2 of 413)	0.0 % (0 of 1)
Methyl	1.1 % (10 of 897)	1.1 % (10 of 897)

1. F1 ATPase beta subunit

MTRGRVIQVM GPVVDVKFEN GHLPAIYNAL KIQHKARNEN EVDIDLTLEV ALHLGDDTVR TIAMASTDGL IRGMEVIDTG APISVPVGQV TLGRVFNVLG EPIDLEGDIP ADARRDPIHR PAPKFEELAT EVEILETGIK VVDLLAPYIK GGKIGLFGGA GVGKTVLIQE LIHNIAQEHG GISVFAGVGE RTREGNDLYH EMKDSGVISK TAMVFGQMNE PPGARMRVAL TGLTMAEYFR DEQGQDGLLF IDNIFRFTQA GSEVSALLGR MPSAIGYQPT LATEMGQLQE RITSTAKGSI TSIQAIYVPA DDYTDPAPAT TFSHLDATTN LERKLAEMGI YPAVDPLVST SRALAPEIVG EEHYQVARKV QQTLERYKEL QDIIAILGMD ELSDEDKLVV HRARRIQFFL SQNFHVAEQF TGQPGSYVPV KETVRGFKEI LEGKYDHLPE DAFRLVGRIE EVVEKAKAMG VEV

2. F1 ATPase alpha subunit

MSIRAEEISA LIKQQIENYE SQIQVSDVGT VIQVGDGIAR AHGLDNVMSG EAVEFANAVM GMALNLEENN VGIVILGPYT GIKEGDEVRR TGRIMEVPVG ETLIGRVVNP LGQPVDGLGP VETTETRPIE SRAPGVMDRR SVHEPLQTGI KAIDALVPIG RGQRELIIGD RQTGKTSVAI DTIINQKDQN MICIYVAIGQ KESTVATVVE TLAKHGAPDY TIVVTASASQ PAPLLFLAPY AGVAMGEYFM IMGKHVLVVI DDLSKQAAAY RQLSLLLRRP PGREAYPGDI FYLHSRLLER AAKLSDAKGG GSLTALPFVE TQAGDISAYI PTNVISITDG QIFLQSDLFF SGVRPAINAG LSVSRVGGAA QIKAMKKVAG TLRLDLAAYR ELEAFAQFGS DLDKATQANV ARGARTVEVL KQDLHQPIPV EKQVLIIYAL TRGFLDDIPV EDVRRFEKEF YLWLDQNGQH LLEHIRTTKD LPNEDDLNQA IEAFKKTFVV SQ

3. F1 ATPase gamma subunit

ASLRDIKTRI NATKKTSQIT KAMEMVLTSK LNRAEKREIV RPYMEKIQEV VANVALAARA SHPMLVSRPV KKTGYLVITS DRGLAGAYNS NVLRLVYQTI QKRHASPDEY AIIVIGRVGL SFFRKRNMPV ILDITRLPDQ PSFADIKEIA RKTVGLFADG TFDELYMYYN HYVSAIQQEV TERKLLPLTD LAENKQRTVY EFEPSQEEIL DVLLPQYAES LIYGALLDAK ASEHAARMTA MKNATDNANE LIRTLTLSYN RARQAAITQE ITEIVAGANA LQ

4. F1 ATPase epsilon subunit

MKTIHVSVVT PDGPVYEDDV EMVSVKAKSG ELGILPGHIP LVAPLEISAA RLKKGGKTQY IAVSGGFLEV RPDNVTILAQ AAERAEDIDV

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 8.0, Details Beta subunit was segmentally labeled (391-473)

#	Name	Isotope labeling	Type	Concentration
1	F1 ATPase beta subunit	[U-100% 13C; U-100% 15N; U-80% 2H]	protein	0.1 mM
2	F1 ATPase alpha subunit	natural abundance	protein	0.1 mM
3	F1 ATPase gamma subunit	natural abundance	protein	0.03 mM
4	F1 ATPase epsilon subunit	natural abundance	protein	0.03 mM
5	Na2SO4		salt	100 mM
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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Release date

2011-06-02

Citation

Analysis of the open and closed conformations of the beta subunits in thermophilic F1-ATPase by solution NMR
Kobayashi, M., Akutsu, H., Suzuki, T., Yoshida, M., Yagi, H.
J. Mol. Biol. (2010), 398, 189-199, PubMed 20230835 , DOI 10.1016/j.jmb.2010.03.013 , Abstract

Related entities 1. F1 ATPase beta subunit, : 80 entities Detail

Related entities 2. F1 ATPase alpha subunit, : 201 entities Detail

Related entities 3. F1 ATPase gamma subunit, : 163 entities Detail

Related entities 4. F1 ATPase epsilon subunit, : 1 : 1 : 10 : 187 entities Detail

Experiments performed 1 experiments Detail

Chemical shift validation 6 contents Detail