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Found 1 Document (1.543 seconds)

BMRB: 11094

2YS5

Solution structure of the complex of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of hALK

Authors

Inoue, M., Kigawa, T., Li, H., Koshiba, S., Yokoyama, S.

Assembly

Fibroblast growth factor receptor substrate 3, ALK tyrosine kinase receptor

Entity

1. PTB domain (polymer, Thiol state: all free), 146 monomers, 16314.11 Da Detail

GSSGSSGLNR DSVPDNHPTK FKVTNVDDEG VELGSGVMEL TQSELVLHLH RREAVRWPYL CLRRYGYDSN LFSFESGRRC QTGQGIFAFK CSRAEEIFNL LQDLMQCNSI NVMEEPVIIT RNSHPAELDL PRAPQPPNAL GYTVSS

2. residues in database 1571-1589 (polymer, Thiol state: all free), 19 monomers, 2340.619 Da Detail

LFRLRHFPCG NVNYGYQQQ

Total weight

18654.729 Da

Max. entity weight

16314.11 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.0 %, Completeness: 87.8 %, Completeness (bb): 86.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.8 % (1679 of 1912)	91.6 % (916 of 1000)	84.1 % (619 of 736)	81.8 % (144 of 176)
Backbone	86.8 % (842 of 970)	91.3 % (304 of 333)	84.4 % (407 of 482)	84.5 % (131 of 155)
Sidechain	88.7 % (970 of 1094)	91.8 % (612 of 667)	85.0 % (345 of 406)	61.9 % (13 of 21)
Aromatic	85.6 % (137 of 160)	98.8 % (79 of 80)	72.2 % (57 of 79)	100.0 % (1 of 1)
Methyl	92.9 % (145 of 156)	93.6 % (73 of 78)	92.3 % (72 of 78)

1. PTB domain

GSSGSSGLNR DSVPDNHPTK FKVTNVDDEG VELGSGVMEL TQSELVLHLH RREAVRWPYL CLRRYGYDSN LFSFESGRRC QTGQGIFAFK CSRAEEIFNL LQDLMQCNSI NVMEEPVIIT RNSHPAELDL PRAPQPPNAL GYTVSS

2. residues in database 1571-1589

LFRLRHFPCG NVNYGYQQQ

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.37mM SNT-2 PTB domain [U-15N, 13C] in complex with hALK peptide (1:1), 20mM d-Tris-HCl, 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	PTB domain	[U-13C; U-15N]	protein	0.37 mM
2	hALK peptide	natural abundance	protein	0.37 mM
3	TRIS	[U-2H]	buffer	20 mM
4	sodium chloride	natural abundance	salt	100 mM
5	DTT	[U-2H]	salt	1 mM
6	sodium azide	natural abundance	salt	0.02 %
7	H2O		solvent	90 %
8	D2O		solvent	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2YS5, Strand ID: A, B Detail

Release date

2011-02-13

Citation

Solution structure of the complex of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of hALK
Li, H., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. PTB domain, : 2 : 10 entities Detail

Related entities 2. residues in database 1571-1589, : 1 : 8 : 3 entities Detail

Interaction partners 1. PTB domain, : 12 interactors Detail

More details for 12 interactors identified by 3 citations

Interaction partners 2. residues in database 1571-1589, : 8 interactors Detail

More details for 8 interactors identified by 7 citations

Experiments performed 5 experiments Detail

1 3D 1H-15N NOESY

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.37mM SNT-2 PTB domain [U-15N, 13C] in complex with hALK peptide (1:1), 20mM d-Tris-HCl, 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	PTB domain	[U-13C; U-15N]	protein	0.37 mM
2	hALK peptide	natural abundance	protein	0.37 mM
3	TRIS	[U-2H]	buffer	20 mM
4	sodium chloride	natural abundance	salt	100 mM
5	DTT	[U-2H]	salt	1 mM
6	sodium azide	natural abundance	salt	0.02 %
7	H2O		solvent	90 %
8	D2O		solvent	10 %

2 3D 1H-13C NOESY

Instrument

Bruker AVANCE - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	PTB domain	[U-13C; U-15N]	protein	0.37 mM
2	hALK peptide	natural abundance	protein	0.37 mM
3	TRIS	[U-2H]	buffer	20 mM
4	sodium chloride	natural abundance	salt	100 mM
5	DTT	[U-2H]	salt	1 mM
6	sodium azide	natural abundance	salt	0.02 %
7	H2O		solvent	90 %
8	D2O		solvent	10 %

3 3D 13C,15N-F1 FILTERED 13C-EDITED NOESY

Instrument

Bruker AVANCE - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	PTB domain	[U-13C; U-15N]	protein	0.37 mM
2	hALK peptide	natural abundance	protein	0.37 mM
3	TRIS	[U-2H]	buffer	20 mM
4	sodium chloride	natural abundance	salt	100 mM
5	DTT	[U-2H]	salt	1 mM
6	sodium azide	natural abundance	salt	0.02 %
7	H2O		solvent	90 %
8	D2O		solvent	10 %

4 3D 13C,15N-F1 FILTERED 15N-EDITED NOESY

Instrument

Bruker AVANCE - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	PTB domain	[U-13C; U-15N]	protein	0.37 mM
2	hALK peptide	natural abundance	protein	0.37 mM
3	TRIS	[U-2H]	buffer	20 mM
4	sodium chloride	natural abundance	salt	100 mM
5	DTT	[U-2H]	salt	1 mM
6	sodium azide	natural abundance	salt	0.02 %
7	H2O		solvent	90 %
8	D2O		solvent	10 %

5 2D F2-13C FILTERED NOESY

Instrument

Bruker AVANCE - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	PTB domain	[U-13C; U-15N]	protein	0.37 mM
2	hALK peptide	natural abundance	protein	0.37 mM
3	TRIS	[U-2H]	buffer	20 mM
4	sodium chloride	natural abundance	salt	100 mM
5	DTT	[U-2H]	salt	1 mM
6	sodium azide	natural abundance	salt	0.02 %
7	H2O		solvent	90 %
8	D2O		solvent	10 %

null

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Found 1 Document (1.543 seconds)

BMRB: 11094

Sample

Related entities 1. PTB domain, : 2 : 10 entities Detail

Related entities 2. residues in database 1571-1589, : 1 : 8 : 3 entities Detail

Interaction partners 1. PTB domain, : 12 interactors Detail

Interaction partners 2. residues in database 1571-1589, : 8 interactors Detail

Experiments performed 5 experiments Detail

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