BMRB: 11146

Solution structure of the eIF-5_eIF-2B domain from human Eukaryotic translation initiation factor 5

Authors

Tomizawa, T., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Eukaryotic translation initiation factor 5

Entity

1. eIF-5 eIF-2B domain (polymer, Thiol state: free and other bound), 157 monomers, 17461.77 Da Detail

GSSGSSGMSV NVNRSVSDQF YRYKMPRLIA KVEGKGNGIK TVIVNMVDVA KALNRPPTYP TKYFGCELGA QTQFDVKNDR YIVNGSHEAN KLQDMLDGFI KKFVLCPECE NPETDLHVNP KKQTIGNSCK ACGYRGMLDT HHKLCTFILK NPPENSD

2. ZN (non-polymer), 65.409 Da

Total weight

17527.178 Da

Max. entity weight

17461.77 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 96.3 %, Completeness (bb): 95.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.3 % (1755 of 1822)	96.3 % (921 of 956)	96.7 % (677 of 700)	94.6 % (157 of 166)
Backbone	95.0 % (878 of 924)	94.0 % (299 of 318)	96.1 % (440 of 458)	93.9 % (139 of 148)
Sidechain	97.6 % (1017 of 1042)	97.5 % (622 of 638)	97.7 % (377 of 386)	100.0 % (18 of 18)
Aromatic	95.2 % (118 of 124)	98.4 % (61 of 62)	91.9 % (57 of 62)
Methyl	100.0 % (144 of 144)	100.0 % (72 of 72)	100.0 % (72 of 72)

1. eIF-5 eIF-2B domain

GSSGSSGMSV NVNRSVSDQF YRYKMPRLIA KVEGKGNGIK TVIVNMVDVA KALNRPPTYP TKYFGCELGA QTQFDVKNDR YIVNGSHEAN KLQDMLDGFI KKFVLCPECE NPETDLHVNP KKQTIGNSCK ACGYRGMLDT HHKLCTFILK NPPENSD

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.32mM eIF-5_eIF-2B domain {U-15N,13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	eIF-5_eIF-2B domain	[U-13C; U-15N]	protein	1.32 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: 2.12 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 2.12 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.12 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 2.52 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2E9H, Strand ID: A Detail

---

Release date

2011-03-31

Citation

Solution structure of the eIF-5_eIF-2B domain from human Eukaryotic translation initiation factor 5
Tomizawa, T., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. eIF-5 eIF-2B domain, : 1 : 7 : 43 entities Detail

Interaction partners 1. eIF-5 eIF-2B domain, : 9 interactors Detail

Experiments performed 2 experiments Detail