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Found 1 Document (6.299 seconds)

BMRB: 11175

2RR8

Solution structure of calponin homology domain of IQGAP1

Authors

Umemoto, R., Nishida, N., Ogino, S., Shimada, I.

Assembly

F-actin binding protein

Entity

1. F-actin binding protein (polymer, Thiol state: all free), 190 monomers, 22005.00 Da Detail

GPLGSLTAEE MDERRRQNVA YEYLCHLEEA KRWMEACLGE DLPPTTELEE GLRNGVYLAK LGNFFSPKVV SLKKIYDREQ TRYKATGLHF RHTDNVIQWL NAMDEIGLPK IFYPETTDIY DRKNMPRCIY CIHALSLYLF KLGLAPQIQD LYGKVDFTEE EINNMKTELE KYGIQMPAFS KIGGILANEL

Formula weight

22005.0 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.6 %, Completeness (bb): 83.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.6 % (1941 of 2295)	89.2 % (1071 of 1201)	76.8 % (688 of 896)	91.9 % (182 of 198)
Backbone	83.5 % (937 of 1122)	96.4 % (370 of 384)	71.6 % (399 of 557)	92.8 % (168 of 181)
Sidechain	87.5 % (1181 of 1350)	85.8 % (701 of 817)	90.3 % (466 of 516)	82.4 % (14 of 17)
Aromatic	53.5 % (106 of 198)	53.5 % (53 of 99)	52.6 % (51 of 97)	100.0 % (2 of 2)
Methyl	100.0 % (206 of 206)	100.0 % (103 of 103)	100.0 % (103 of 103)

1. CH domain

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	CH domain	[U-99% 13C; U-99% 15N]	0.90 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 mM
6	H2O		90 %
7	D2O		10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
8	CH domain	[U-99% 13C; U-99% 15N]	0.90 mM
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	150 mM
11	DTT	natural abundance	1 mM
12	sodium azide	natural abundance	1 mM
13	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	internal	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: 2.46 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	internal	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: 2.46 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	internal	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RR8, Strand ID: A Detail

Release date

2010-09-08

Citation

NMR structure of the calponin homology domain of human IQGAP1 and its implications for the actin recognition mode
Umemoto, R., Nishida, N., Ogino, S., Shimada, I.
J. Biomol. NMR (2010), 48, 59-64, PubMed 20644981 , DOI 10.1007/s10858-010-9434-8

Related entities 1. F-actin binding protein, : 1 : 7 : 27 entities Detail

Interaction partners 1. F-actin binding protein, : 126 interactors Detail

More details for 126 interactors identified by 52 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	CH domain	[U-99% 13C; U-99% 15N]	0.90 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 mM
6	H2O		90 %
7	D2O		10 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
8	CH domain	[U-99% 13C; U-99% 15N]	0.90 mM
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	150 mM
11	DTT	natural abundance	1 mM
12	sodium azide	natural abundance	1 mM
13	D2O		100 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	CH domain	[U-99% 13C; U-99% 15N]	0.90 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 mM
6	H2O		90 %
7	D2O		10 %

4 3D C(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	CH domain	[U-99% 13C; U-99% 15N]	0.90 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 mM
6	H2O		90 %
7	D2O		10 %

5 3D HNCA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	CH domain	[U-99% 13C; U-99% 15N]	0.90 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 mM
6	H2O		90 %
7	D2O		10 %

6 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	CH domain	[U-99% 13C; U-99% 15N]	0.90 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 mM
6	H2O		90 %
7	D2O		10 %

7 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
8	CH domain	[U-99% 13C; U-99% 15N]	0.90 mM
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	150 mM
11	DTT	natural abundance	1 mM
12	sodium azide	natural abundance	1 mM
13	D2O		100 %

8 3D HCCH-COSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
8	CH domain	[U-99% 13C; U-99% 15N]	0.90 mM
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	150 mM
11	DTT	natural abundance	1 mM
12	sodium azide	natural abundance	1 mM
13	D2O		100 %

9 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
8	CH domain	[U-99% 13C; U-99% 15N]	0.90 mM
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	150 mM
11	DTT	natural abundance	1 mM
12	sodium azide	natural abundance	1 mM
13	D2O		100 %

10 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	CH domain	[U-99% 13C; U-99% 15N]	0.90 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 mM
6	H2O		90 %
7	D2O		10 %

11 3D HNHA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
14	CH domain	[U-99% 15N]	0.90 mM
15	sodium phosphate	natural abundance	20 mM
16	sodium chloride	natural abundance	150 mM
17	DTT	natural abundance	1 mM
18	sodium azide	natural abundance	1 mM
19	H2O		90 %
20	D2O		10 %

12 3D H(CCO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	CH domain	[U-99% 13C; U-99% 15N]	0.90 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 mM
6	H2O		90 %
7	D2O		10 %

13 3D HN(CO)CA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	CH domain	[U-99% 13C; U-99% 15N]	0.90 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 mM
6	H2O		90 %
7	D2O		10 %

14 3D HBHA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	CH domain	[U-99% 13C; U-99% 15N]	0.90 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 mM
6	H2O		90 %
7	D2O		10 %

15 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	CH domain	[U-99% 13C; U-99% 15N]	0.90 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	1 mM
6	H2O		90 %
7	D2O		10 %

nullKeywords F-actin binding protein

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Found 1 Document (6.299 seconds)

BMRB: 11175

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. F-actin binding protein, : 1 : 7 : 27 entities Detail

Interaction partners 1. F-actin binding protein, : 126 interactors Detail

Experiments performed 15 experiments Detail

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