BMRB: 11178

Solution structure of the SH3 domain of human olygophrein-1 like protein (KIAA0621)

Authors

Inoue, K., Hayashi, F., Shirouzu, M., Terada, T., Kigawa, T., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Matsuo, Y., Ohara, O., Nagase, T., Kikuno, R., Nakayama, M., Yokoyama, S.

Assembly

Olygophrenin-1 like protein

Entity

1. Olygophrenin-1 like protein (polymer, Thiol state: all free), 72 monomers, 7518.127 Da Detail

GSSGSSGTPF RKAKALYACK AEHDSELSFT AGTVFDNVHP SQEPGWLEGT LNGKTGLIPE NYVEFLSGPS SG

Formula weight

7518.127 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.7 %, Completeness: 93.1 %, Completeness (bb): 89.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.1 % (725 of 779)	93.8 % (376 of 401)	93.1 % (285 of 306)	88.9 % (64 of 72)
Backbone	89.3 % (377 of 422)	89.3 % (133 of 149)	89.8 % (185 of 206)	88.1 % (59 of 67)
Sidechain	96.9 % (406 of 419)	96.4 % (243 of 252)	97.5 % (158 of 162)	100.0 % (5 of 5)
Aromatic	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (37 of 37)	100.0 % (1 of 1)
Methyl	100.0 % (60 of 60)	100.0 % (30 of 30)	100.0 % (30 of 30)

1. SH3 domain

GSSGSSGTPF RKAKALYACK AEHDSELSFT AGTVFDNVHP SQEPGWLEGT LNGKTGLIPE NYVEFLSGPS SG

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.2mM SH3 domain {U-15N,13C;} 20mM Tris buffer {NA;} 100mM {NaCl;} 1mM {d10-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1.2 mM
2	Tris	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d10-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.46 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UGV, Strand ID: A Detail

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Release date

2011-07-19

Citation

Solution structure of the SH3 domain of human olygophrein-1 like protein (KIAA0621)
Inoue, K., Hayashi, F., Shirouzu, M., Terada, T., Kigawa, T., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Matsuo, Y., Ohara, O., Nagase, T., Kikuno, R., Nakayama, M., Yokoyama, S.

Related entities 1. Olygophrenin-1 like protein, : 1 : 2 : 169 entities Detail

Interaction partners 1. Olygophrenin-1 like protein, : 8 interactors Detail

Experiments performed 2 experiments Detail