BMRB: 11198

Solution structure of the third PDZ domain of synapse-associated protein 102

Authors

Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

synapse-associated protein 102

Entity

1. synapse-associated protein 102 (polymer, Thiol state: not present), 113 monomers, 11639.70 Da Detail

GSSGSSGRPG GDAREPRKII LHKGSTGLGF NIVGGEDGEG IFVSFILAGG PADLSGELRR GDRILSVNGV NLRNATHEQA AAALKRAGQS VTIVAQYRPE EYSRFESSGP SSG

Formula weight

11639.7 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.4 %, Completeness (bb): 98.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	98.4 % (1181 of 1200)	97.6 % (609 of 624)	99.6 % (459 of 461)	98.3 % (113 of 115)
Backbone	98.5 % (658 of 668)	97.5 % (235 of 241)	99.4 % (317 of 319)	98.1 % (106 of 108)
Sidechain	98.6 % (616 of 625)	97.7 % (374 of 383)	100.0 % (235 of 235)	100.0 % (7 of 7)
Aromatic	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)
Methyl	100.0 % (110 of 110)	100.0 % (55 of 55)	100.0 % (55 of 55)

1. PDZ domain

GSSGSSGRPG GDAREPRKII LHKGSTGLGF NIVGGEDGEG IFVSFILAGG PADLSGELRR GDRILSVNGV NLRNATHEQA AAALKRAGQS VTIVAQYRPE EYSRFESSGP SSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05), Details 1.05mM 13C, 15N-labeled {protein;} 20mM phosphate {buffer;} 100mM {NaCl;} 1mM {d10-DTT;} 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]	protein	1.05 mM
2	phosphate	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.11 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UM7, Strand ID: A Detail

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Release date

2011-07-19

Citation

Solution structure of the third PDZ domain of synapse-associated protein 102
Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. synapse-associated protein 102, : 1 : 7 : 198 entities Detail

Interaction partners 1. synapse-associated protein 102, : 24 interactors Detail

Experiments performed 2 experiments Detail