BMRB: 11245

Solution Structure of the murine ubiquitin-like 5 protein from RIKEN cDNA 0610031K06

Authors

Hayashi, F., Shirouzu, M., Terada, T., Kigawa, T., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Arakawa, T., Carninci, P., Kawai, J., Hayashizaki, Y., Yokoyama, S.

Assembly

ubiquitin-like 5

Entity

1. ubiquitin-like 5 (polymer, Thiol state: all free), 100 monomers, 11288.74 Da Detail

MKGSSHHHHH HSSGASLVPR GSEGAATMIE VVCNDRLGKK VRVKCNTDDT IGDLKKLIAA QTGTRWNKIV LKKWYTIFKD HVSLGDYEIH DGMNLELYYQ

Formula weight

11288.74 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.0 %, Completeness: 92.2 %, Completeness (bb): 92.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.2 % (1069 of 1159)	92.7 % (556 of 600)	91.8 % (415 of 452)	91.6 % (98 of 107)
Backbone	92.5 % (553 of 598)	93.3 % (194 of 208)	92.4 % (269 of 291)	90.9 % (90 of 99)
Sidechain	92.0 % (600 of 652)	92.3 % (362 of 392)	91.3 % (230 of 252)	100.0 % (8 of 8)
Aromatic	71.4 % (70 of 98)	71.4 % (35 of 49)	70.2 % (33 of 47)	100.0 % (2 of 2)
Methyl	100.0 % (106 of 106)	100.0 % (53 of 53)	100.0 % (53 of 53)

1. ubiquitin-like 5

MKGSSHHHHH HSSGASLVPR GSEGAATMIE VVCNDRLGKK VRVKCNTDDT IGDLKKLIAA QTGTRWNKIV LKKWYTIFKD HVSLGDYEIH DGMNLELYYQ

Show sample condition

Sample

Solvent system 92% H2O/8% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05), Details 0.5mM 13C, 15N-labeled {protein;} 20mM phosphate {buffer;} 500mM {NaCl;} 4mM {DTT;} 0.4mM {NaN3;} 8% D2O

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-like 5	[U-13C; U-15N]	protein	0.5 mM
2	phosphate	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	500 mM
4	DTT	natural abundance	salt	4 mM
5	NaN3	natural abundance	salt	0.4 mM
6	H2O		solvent	92 %
7	D2O		solvent	8 %

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LACS Plot; CA

Referencing offset: -0.11 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.11 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 6 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UH6, Strand ID: A Detail

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Release date

2011-08-02

Citation

Solution Structure of the murine ubiquitin-like 5 protein from RIKEN cDNA 0610031K06
Hayashi, F., Shirouzu, M., Terada, T., Kigawa, T., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Arakawa, T., Carninci, P., Kawai, J., Hayashizaki, Y., Yokoyama, S.

Related entities 1. ubiquitin-like 5, : 1 : 12 entities Detail

Experiments performed 2 experiments Detail