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Solution Structure of the murine ubiquitin-like 5 protein from RIKEN cDNA 0610031K06
Authors
Hayashi, F., Shirouzu, M., Terada, T., Kigawa, T., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Arakawa, T., Carninci, P., Kawai, J., Hayashizaki, Y., Yokoyama, S.
Assembly
ubiquitin-like 5
Entity
1. ubiquitin-like 5 (polymer, Thiol state: all free), 100 monomers, 11288.74 Da Detail

MKGSSHHHHH HSSGASLVPR GSEGAATMIE VVCNDRLGKK VRVKCNTDDT IGDLKKLIAA QTGTRWNKIV LKKWYTIFKD HVSLGDYEIH DGMNLELYYQ


Formula weight
11288.74 Da
Source organism
Mus musculus
Exptl. method
solution NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.0 %, Completeness: 92.8 %, Completeness (bb): 92.7 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All92.8 % (1079 of 1163)93.3 % (560 of 600)92.7 % (419 of 452)90.1 % (100 of 111)
Backbone92.7 % (546 of 589)93.0 % (185 of 199)93.5 % (272 of 291)89.9 % (89 of 99)
Sidechain92.9 % (618 of 665)93.5 % (375 of 401)92.1 % (232 of 252)91.7 % (11 of 12)
Aromatic73.5 % (72 of 98)75.5 % (37 of 49)70.2 % (33 of 47)100.0 % (2 of 2)
Methyl100.0 % (106 of 106)100.0 % (53 of 53)100.0 % (53 of 53)

1. ubiquitin-like 5

MKGSSHHHHH HSSGASLVPR GSEGAATMIE VVCNDRLGKK VRVKCNTDDT IGDLKKLIAA QTGTRWNKIV LKKWYTIFKD HVSLGDYEIH DGMNLELYYQ

Sample

Solvent system 92% H2O/8% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05), Details 0.5mM 13C, 15N-labeled {protein;} 20mM phosphate {buffer;} 500mM {NaCl;} 4mM {DTT;} 0.4mM {NaN3;} 8% D2O


#NameIsotope labelingTypeConcentration
1ubiquitin-like 5[U-13C; U-15N]protein0.5 mM
2phosphatenatural abundancebuffer20 mM
3NaClnatural abundancesalt500 mM
4DTTnatural abundancesalt4 mM
5NaN3natural abundancesalt0.4 mM
6H2Osolvent92 %
7D2Osolvent8 %

LACS Plot; CA
Referencing offset: -0.11 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: -0.11 ppm, Outliers: 3 Detail
LACS Plot; HA
Referencing offset: -0.06 ppm, Outliers: 6 Detail
LACS Plot; CO
Referencing offset: 0.07 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 1UH6, Strand ID: A Detail


Release date
2011-08-02
Citation
Solution Structure of the murine ubiquitin-like 5 protein from RIKEN cDNA 0610031K06
Hayashi, F., Shirouzu, M., Terada, T., Kigawa, T., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Arakawa, T., Carninci, P., Kawai, J., Hayashizaki, Y., Yokoyama, S.
Related entities 1. ubiquitin-like 5, : 1 : 7 entities Detail