BMRB: 11247

Solution structure of the FHA domain of human ubiquitin ligase protein RNF8

Authors

Hayashi, F., Nagashima, T., Yokoyama, S.

Assembly

Ubiquitin ligase protein RNF8

Entity

1. Ubiquitin ligase protein RNF8 (polymer, Thiol state: all free), 145 monomers, 16039.90 Da Detail

GSSGSSGVTG DRAGGRSWCL RRVGMSAGWL LLEDGCEVTV GRGFGVTYQL VSKICPLMIS RNHCVLKQNP EGQWTIMDNK SLNGVWLNRA RLEPLRVYSI HQGDYIQLGV PLENKENAEY EYEVTEEDWE TIYPCLSPKS GPSSG

Formula weight

16039.9 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 95.6 %, Completeness (bb): 94.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.6 % (1571 of 1643)	95.6 % (817 of 855)	96.1 % (608 of 633)	94.2 % (146 of 155)
Backbone	94.3 % (807 of 856)	93.4 % (281 of 301)	95.2 % (397 of 417)	93.5 % (129 of 138)
Sidechain	96.2 % (879 of 914)	95.5 % (529 of 554)	97.1 % (333 of 343)	100.0 % (17 of 17)
Aromatic	98.4 % (124 of 126)	100.0 % (63 of 63)	96.6 % (56 of 58)	100.0 % (5 of 5)
Methyl	95.1 % (137 of 144)	93.1 % (67 of 72)	97.2 % (70 of 72)

1. FHA domain

GSSGSSGVTG DRAGGRSWCL RRVGMSAGWL LLEDGCEVTV GRGFGVTYQL VSKICPLMIS RNHCVLKQNP EGQWTIMDNK SLNGVWLNRA RLEPLRVYSI HQGDYIQLGV PLENKENAEY EYEVTEEDWE TIYPCLSPKS GPSSG

Show sample condition

Sample

Solvent system 90% {H2O;} 10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.1mM 13C,15N-labeled {protein;} 20mM {d-TrisHCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	FHA domain	[U-13C; U-15N]	protein	1.1 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.42 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2CSW, Strand ID: A Detail

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Release date

2011-08-02

Citation

Solution structure of the FHA domain of human ubiquitin ligase protein RNF8
Hayashi, F., Nagashima, T., Yokoyama, S.

Related entities 1. Ubiquitin ligase protein RNF8, : 1 : 13 entities Detail

Interaction partners 1. Ubiquitin ligase protein RNF8, : 32 interactors Detail

Experiments performed 2 experiments Detail