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Solution structure of the FHA domain of human ubiquitin ligase protein RNF8
Authors
Hayashi, F., Nagashima, T., Yokoyama, S.
Assembly
Ubiquitin ligase protein RNF8
Entity
1. Ubiquitin ligase protein RNF8 (polymer, Thiol state: all free), 145 monomers, 16039.90 Da Detail

GSSGSSGVTG DRAGGRSWCL RRVGMSAGWL LLEDGCEVTV GRGFGVTYQL VSKICPLMIS RNHCVLKQNP EGQWTIMDNK SLNGVWLNRA RLEPLRVYSI HQGDYIQLGV PLENKENAEY EYEVTEEDWE TIYPCLSPKS GPSSG


Formula weight
16039.9 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.9 %, Completeness: 95.4 %, Completeness (bb): 93.8 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All95.4 % (1576 of 1652)96.0 % (821 of 855)96.1 % (608 of 633)89.6 % (147 of 164)
Backbone93.8 % (786 of 838)94.0 % (266 of 283)94.5 % (394 of 417)91.3 % (126 of 138)
Sidechain96.9 % (912 of 941)97.0 % (555 of 572)98.0 % (336 of 343)80.8 % (21 of 26)
Aromatic98.4 % (124 of 126)100.0 % (63 of 63)96.6 % (56 of 58)100.0 % (5 of 5)
Methyl100.0 % (144 of 144)100.0 % (72 of 72)100.0 % (72 of 72)

1. FHA domain

GSSGSSGVTG DRAGGRSWCL RRVGMSAGWL LLEDGCEVTV GRGFGVTYQL VSKICPLMIS RNHCVLKQNP EGQWTIMDNK SLNGVWLNRA RLEPLRVYSI HQGDYIQLGV PLENKENAEY EYEVTEEDWE TIYPCLSPKS GPSSG

Sample

Solvent system 90% {H2O;} 10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.1mM 13C,15N-labeled {protein;} 20mM {d-TrisHCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3


#NameIsotope labelingTypeConcentration
1FHA domain[U-13C; U-15N]protein1.1 mM
2d-Tris-HClnatural abundancebuffer20 mM
3NaClnatural abundancesalt100 mM
4d-DTTnatural abundancesalt1 mM
5NaN3natural abundancesalt0.02 %
6H2Osolvent90 %
7D2Osolvent10 %

LACS Plot; CA
Referencing offset: 0.14 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.14 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.03 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.42 ppm, Outliers: 4 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2CSW, Strand ID: A Detail


Release date
2011-08-02
Citation
Solution structure of the FHA domain of human ubiquitin ligase protein RNF8
Hayashi, F., Nagashima, T., Yokoyama, S.
Related entities 1. Ubiquitin ligase protein RNF8, : 1 : 9 entities Detail
Interaction partners 1. Ubiquitin ligase protein RNF8, : 31 interactors Detail