BMRB: 11264

Solution Structure of Ras-binding Domain in Mouse AF-6 Protein

Authors

Zhao, C., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Afadin

Entity

1. Afadin (polymer, Thiol state: all free), 116 monomers, 12598.02 Da Detail

GSSGSSGSGG TLRIYADSLK PNIPYKTILL STTDTADFAV AESLEKYGLE KENPKDYCIA RVMLPPGAQH SDERGAKEII LDDDECPLQI FREWPSDKGI LVFQLKRRPP SGPSSG

Formula weight

12598.02 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.4 %, Completeness: 92.5 %, Completeness (bb): 89.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.5 % (1213 of 1312)	92.4 % (636 of 688)	93.0 % (476 of 512)	90.2 % (101 of 112)
Backbone	89.8 % (607 of 676)	88.8 % (207 of 233)	90.5 % (305 of 337)	89.6 % (95 of 106)
Sidechain	95.0 % (704 of 741)	94.3 % (429 of 455)	96.1 % (269 of 280)	100.0 % (6 of 6)
Aromatic	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (38 of 38)	100.0 % (1 of 1)
Methyl	100.0 % (112 of 112)	100.0 % (56 of 56)	100.0 % (56 of 56)

1. Ras-binding domain

GSSGSSGSGG TLRIYADSLK PNIPYKTILL STTDTADFAV AESLEKYGLE KENPKDYCIA RVMLPPGAQH SDERGAKEII LDDDECPLQI FREWPSDKGI LVFQLKRRPP SGPSSG

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Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 298.0 (±0.1) K, pH 7.0 (±0.05), Details 1.28mM protein U-15N, {13C;} 20mM d-Tris-HCl {(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Ras-binding domain	[U-13C; U-15N]	protein	1.28 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.2 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WXA, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution Structure of Ras-binding Domain in Mouse AF-6 Protein
Zhao, C., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Afadin, : 1 : 4 : 21 entities Detail

Interaction partners 1. Afadin, : 4 interactors Detail

Experiments performed 2 experiments Detail