BMRB: 11330

Solution structure of the plus-3 domain of human KIAA0252 protein

Authors

Yoneyama, M., Kigawa, T., Tochio, N., Nameki, N., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Paf1/RNA polymerase II complex component

Entity

1. Paf1/RNA polymerase II complex component (polymer, Thiol state: all free), 149 monomers, 16639.75 Da Detail

GSSGSSGPPK SQPVSLPEEL NRVRLSRHKL ERWCHMPFFA KTVTGCFVRI GIGNHNSKPV YRVAEITGVV ETAKVYQLGG TRTNKGLQLR HGNDQRVFRL EFVSNQEFTE SEFMKWKEAM FSAGMQLPTL DEINKKELSI KEASGPSSG

Formula weight

16639.75 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.3 %, Completeness: 93.6 %, Completeness (bb): 91.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.6 % (1636 of 1748)	93.0 % (855 of 919)	94.9 % (639 of 673)	91.0 % (142 of 156)
Backbone	91.6 % (804 of 878)	89.5 % (272 of 304)	93.3 % (404 of 433)	90.8 % (128 of 141)
Sidechain	95.8 % (963 of 1005)	94.8 % (583 of 615)	97.6 % (366 of 375)	93.3 % (14 of 15)
Aromatic	94.9 % (129 of 136)	95.6 % (65 of 68)	93.9 % (62 of 66)	100.0 % (2 of 2)
Methyl	99.3 % (135 of 136)	98.5 % (67 of 68)	100.0 % (68 of 68)

1. Plus-3 domain, Residues 8-143

GSSGSSGPPK SQPVSLPEEL NRVRLSRHKL ERWCHMPFFA KTVTGCFVRI GIGNHNSKPV YRVAEITGVV ETAKVYQLGG TRTNKGLQLR HGNDQRVFRL EFVSNQEFTE SEFMKWKEAM FSAGMQLPTL DEINKKELSI KEASGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 0.73mM plus-3 domain {U-15N,13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	plus-3 domain	[U-13C; U-15N]	protein	0.73 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.24 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DB9, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the plus-3 domain of human KIAA0252 protein
Yoneyama, M., Kigawa, T., Tochio, N., Nameki, N., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Paf1/RNA polymerase II complex component, : 1 : 9 : 30 entities Detail

Interaction partners 1. Paf1/RNA polymerase II complex component, : 1 interactors Detail

Experiments performed 2 experiments Detail