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Assigned chemical shifts of the zf-CW domain with H3 peptide
Authors
He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.
Assembly
Zinc finger CW-type PWWP domain protein 1
Entity
1. zf-CW domain (polymer, Thiol state: free and other bound), 69 monomers, 7644.315 Da Detail

GSSGSSGEIS GFGQCLVWVQ CSFPNCGKWR RLCGNIDPSV LPDNWSCDQN TDVQYNRCDI PEETWTGLE


2. ZN (non-polymer), 65.409 Da
3. H3 peptide (polymer, Thiol state: not present), 20 monomers, 2183.515 Da Detail

ARTKQTARKS TGGKAPRKQL


Total weight
9893.239 Da
Max. entity weight
7644.315 Da
Entity Connection
na 4 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1nasing1:CYS21:SG2:ZN1:ZN
2nasing1:CYS26:SG2:ZN1:ZN
3nasing1:CYS47:SG2:ZN1:ZN
4nasing1:CYS58:SG2:ZN1:ZN

Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
Torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 66.3 %, Completeness: 21.6 %, Completeness (bb): 44.7 % Detail

Polymer type: polypeptide(L)

Total1H15N
All21.6 % (136 of 631)14.6 % (77 of 526)56.2 % (59 of 105)
Backbone44.7 % (115 of 257)32.4 % (56 of 173)70.2 % (59 of 84)
Sidechain 5.6 % (21 of 374) 5.9 % (21 of 353) 0.0 % (0 of 21)
Aromatic 9.5 % (4 of 42)10.5 % (4 of 38) 0.0 % (0 of 4)
Methyl 9.1 % (3 of 33) 9.1 % (3 of 33)

1. zf-CW domain

GSSGSSGEIS GFGQCLVWVQ CSFPNCGKWR RLCGNIDPSV LPDNWSCDQN TDVQYNRCDI PEETWTGLE

3. H3 peptide

ARTKQTARKS TGGKAPRKQL

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 20mM d-Tris-HCl(pH {7.0);} 300uM {H3 peptide;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 50uM ZnCl2+1mM {IDA;} 10% D2O, 90% H2O


#NameIsotope labelingTypeConcentration
1zf-CW domainprotein0.0 ~ 0.0 mM
2H3 peptideprotein300 uM
3d-Tris-HClnatural abundancebuffer20 mM
4NaClnatural abundancesalt100 mM
5d-DTTnatural abundancesalt1 mM
6NaN3natural abundancesalt0.02 %
7ZnCl2natural abundancesalt50 uM
8IDAnatural abundancesalt1 mM
9H2Osolvent90 %
10D2Osolvent10 %

Protein Blocks Logo
Calculated from 20 models in PDB: 2E61, Strand ID: A Detail


Release date
2011-01-03
Citation
Structural insight into the zinc finger CW domain as a histone modification reader
He, F., Umehara, T., Saito, K., Harada, T., Watanabe, S., Yabuki, T., Kigawa, T., Takahashi, M., Kuwasako, K., Tsuda, K., Matsuda, T., Aoki, M., Seki, E., Kobayashi, N., Guntert, P., Yokoyama, S., Muto, Y.
Structure (2010), 18, 1127-1139, PubMed 20826339 , DOI 10.1016/j.str.2010.06.012 ,
Related entities 1. zf-CW domain, : 1 : 1 : 1 : 3 entities Detail
Related entities 3. H3 peptide, : 46 : 2 : 15 : 11 entities Detail
Interaction partners 1. zf-CW domain, : 3 interactors Detail
Interaction partners 3. H3 peptide, : 2 interactors Detail