BMRB: 11358

Assigned chemical shifts of the zf-CW domain with H3 peptide

Authors

He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Assembly

Zinc finger CW-type PWWP domain protein 1

Entity

1. zf-CW domain (polymer, Thiol state: free and other bound), 69 monomers, 7644.315 Da Detail

GSSGSSGEIS GFGQCLVWVQ CSFPNCGKWR RLCGNIDPSV LPDNWSCDQN TDVQYNRCDI PEETWTGLE

2. ZN (non-polymer), 65.409 Da
3. H3 peptide (polymer, Thiol state: not present), 20 monomers, 2183.515 Da Detail

ARTKQTARKS TGGKAPRKQL

Total weight

9893.239 Da

Max. entity weight

7644.315 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS21:SG	2:ZN1:ZN
2	na	sing	1:CYS26:SG	2:ZN1:ZN
3	na	sing	1:CYS47:SG	2:ZN1:ZN
4	na	sing	1:CYS58:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 64.0 %, Completeness: 19.2 %, Completeness (bb): 43.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	19.2 % (120 of 625)	12.0 % (63 of 526)	57.6 % (57 of 99)
Backbone	43.4 % (116 of 267)	32.2 % (59 of 183)	67.9 % (57 of 84)
Sidechain	1.1 % (4 of 358)	1.2 % (4 of 343)	0.0 % (0 of 15)
Aromatic	0.0 % (0 of 42)	0.0 % (0 of 38)	0.0 % (0 of 4)
Methyl	0.0 % (0 of 33)	0.0 % (0 of 33)

1. zf-CW domain

GSSGSSGEIS GFGQCLVWVQ CSFPNCGKWR RLCGNIDPSV LPDNWSCDQN TDVQYNRCDI PEETWTGLE

3. H3 peptide

ARTKQTARKS TGGKAPRKQL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 20mM d-Tris-HCl(pH {7.0);} 300uM {H3 peptide;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 50uM ZnCl2+1mM {IDA;} 10% D2O, 90% H2O

#	Name	Isotope labeling	Type	Concentration
1	zf-CW domain		protein	0.0 ~ 0.0 mM
2	H3 peptide		protein	300 uM
3	d-Tris-HCl	natural abundance	buffer	20 mM
4	NaCl	natural abundance	salt	100 mM
5	d-DTT	natural abundance	salt	1 mM
6	NaN3	natural abundance	salt	0.02 %
7	ZnCl2	natural abundance	salt	50 uM
8	IDA	natural abundance	salt	1 mM
9	H2O		solvent	90 %
10	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2E61, Strand ID: A Detail

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Release date

2011-01-03

Citation

Structural insight into the zinc finger CW domain as a histone modification reader
He, F., Umehara, T., Saito, K., Harada, T., Watanabe, S., Yabuki, T., Kigawa, T., Takahashi, M., Kuwasako, K., Tsuda, K., Matsuda, T., Aoki, M., Seki, E., Kobayashi, N., Guntert, P., Yokoyama, S., Muto, Y.
Structure (2010), 18, 1127-1139, PubMed 20826339 , DOI 10.1016/j.str.2010.06.012 , Abstract

Entries sharing articles BMRB: 5 entries Detail

Related entities 1. zf-CW domain, : 1 : 1 : 2 : 10 entities Detail

Related entities 3. H3 peptide, : 90 : 2 : 30 : 22 entities Detail

Interaction partners 1. zf-CW domain, : 3 interactors Detail

Interaction partners 3. H3 peptide, : 2 interactors Detail

Experiments performed 2 experiments Detail