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Assigned chemical shifts of the zf-CW domain with H4 trimethyl K20 peptide
Authors
He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.
Assembly
Zinc finger CW-type PWWP domain protein 1
Entity
1. zf-CW domain (polymer, Thiol state: free and other bound), 69 monomers, 7644.315 Da Detail

GSSGSSGEIS GFGQCLVWVQ CSFPNCGKWR RLCGNIDPSV LPDNWSCDQN TDVQYNRCDI PEETWTGLE


2. ZN (non-polymer), 65.409 Da
3. H4 trimethyl K20 peptide (polymer, Thiol state: not present), 20 monomers, 2035.385 Da Detail

SGRGKGGKGL GKGGAKRHRX


Total weight
9745.109 Da
Max. entity weight
7644.315 Da
Entity Connection
na 4 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1nasing1:CYS21:SG2:ZN1:ZN
2nasing1:CYS26:SG2:ZN1:ZN
3nasing1:CYS47:SG2:ZN1:ZN
4nasing1:CYS58:SG2:ZN1:ZN

Source organism
Homo sapiens
Exptl. method
SOLUTION NMR
Refine. method
Torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 64.0 %, Completeness: 19.4 %, Completeness (bb): 42.6 % Detail

Polymer type: polypeptide(L)

Total1H15N
All19.4 % (119 of 612)12.2 % (62 of 509)55.3 % (57 of 103)
Backbone42.6 % (109 of 256)30.2 % (52 of 172)67.9 % (57 of 84)
Sidechain 2.8 % (10 of 356) 3.0 % (10 of 337) 0.0 % (0 of 19)
Aromatic 0.0 % (0 of 44) 0.0 % (0 of 40) 0.0 % (0 of 4)
Methyl 0.0 % (0 of 28) 0.0 % (0 of 28)

1. zf-CW domain

GSSGSSGEIS GFGQCLVWVQ CSFPNCGKWR RLCGNIDPSV LPDNWSCDQN TDVQYNRCDI PEETWTGLE

3. H4 trimethyl K20 peptide

SGRGKGGKGL GKGGAKRHRX

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 20mM d-Tris-HCl(pH {7.0);} 300uM {H4 peptide;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 50uM ZnCl2+1mM {IDA;} 10% D2O, 90% H2O


#NameIsotope labelingTypeConcentration
1zf-CW domainprotein0.0 ~ 0.0 mM
2H4 trimethyl K20 peptideprotein0.0 ~ 0.0 mM
3d-Tris-HClnatural abundancebuffer20 mM
4NaClnatural abundancesalt100 mM
5d-DTTnatural abundancesalt1 mM
6NaN3natural abundancesalt0.02 %
7ZnCl2natural abundancesalt50 uM
8IDAnatural abundancesalt1 mM
9H2Osolvent90 %
10D2Osolvent10 %

Protein Blocks Logo
Calculated from 20 models in PDB: 2E61, Strand ID: A Detail


Release date
2011-01-03
Citation
Structural insight into the zinc finger CW domain as a histone modification reader
He, F., Umehara, T., Saito, K., Harada, T., Watanabe, S., Yabuki, T., Kigawa, T., Takahashi, M., Kuwasako, K., Tsuda, K., Matsuda, T., Aoki, M., Seki, E., Kobayashi, N., Guntert, P., Yokoyama, S., Muto, Y.
Structure (2010), 18, 1127-1139, PubMed 20826339 , DOI 10.1016/j.str.2010.06.012 ,
Entries sharing articles BMRB: 5 entries Detail
  BMRB: 11358 released on 2011-01-03
    Title Assigned chemical shifts of the zf-CW domain with H3 peptide
  BMRB: 11359 released on 2011-01-03
    Title Assigned chemical shifts of the zf-CW domain with H3 dimethyl K4 peptide
  BMRB: 11360 released on 2011-01-03
    Title Assigned chemical shifts of the zf-CW domain with H3 trimethyl K4 peptide
  BMRB: 11362 released on 2011-01-03
    Title Assigned chemical shifts of the zf-CW domain in zinc finger CW-type PWWP domain protein 1
  BMRB: 11115 released on 2010-09-30
    Title Complex structure of the zf-CW domain and the H3K4me3 peptide
Related entities 1. zf-CW domain, : 1 : 1 : 1 : 3 entities Detail
Related entities 3. H4 trimethyl K20 peptide, : 128 : 1 entities Detail
Interaction partners 1. zf-CW domain, : 3 interactors Detail
Interaction partners 3. H4 trimethyl K20 peptide, : 254 interactors Detail
Experiments performed 2 experiments Detail