BMRB: 11473

Solution structure of the CERT PH domain

Authors

Sugiki, T., Takeuchi, K., Tokunaga, Y., Kumagai, K., Kawano, M., Nishijima, M., Hanada, K., Takahashi, H., Shimada, I.

Assembly

CERT PH domain

Entity

1. CERT PH domain (polymer, Thiol state: all free), 94 monomers, 11165.26 Da Detail

VERCGVLSKW TNYIHGWQDR WVVLKNNALS YYKSEDETEY GCRGSICLSK AVITPHDFDE CRFDISVNDS VWYLRAQDPD HRQQWIDAIE QHKT

Formula weight

11165.26 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 83.9 %, Completeness (bb): 89.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	83.9 % (941 of 1122)	82.5 % (480 of 582)	83.6 % (363 of 434)	92.5 % (98 of 106)
Backbone	89.5 % (501 of 560)	86.8 % (165 of 190)	90.6 % (252 of 278)	91.3 % (84 of 92)
Sidechain	80.4 % (524 of 652)	80.4 % (315 of 392)	79.3 % (195 of 246)	100.0 % (14 of 14)
Aromatic	73.5 % (100 of 136)	89.7 % (61 of 68)	54.0 % (34 of 63)	100.0 % (5 of 5)
Methyl	85.2 % (75 of 88)	84.1 % (37 of 44)	86.4 % (38 of 44)

1. CERT

VERCGVLSKW TNYIHGWQDR WVVLKNNALS YYKSEDETEY GCRGSICLSK AVITPHDFDE CRFDISVNDS VWYLRAQDPD HRQQWIDAIE QHKT

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 7.20

#	Name	Isotope labeling	Type	Concentration
1	CERT	[U-98% 15N]	protein	0.2 mM
2	HEPES	natural abundance		10 mM
3	sodium chloride	natural abundance		100 mM
4	DTT	natural abundance		5 mM
5	H2O		solvent	93 %
6	D2O		solvent	7 %

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 7.20

#	Name	Isotope labeling	Type	Concentration
7	CERT	[U-98% 13C; U-98% 15N]		0.2 mM
8	HEPES	natural abundance		10 mM
9	sodium chloride	natural abundance		100 mM
10	DTT	natural abundance		5 mM
11	H2O		solvent	93 %
12	D2O		solvent	7 %

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LACS Plot; CA

Referencing offset: 0.22 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.22 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.36 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RSG, Strand ID: A Detail

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Release date

2012-08-12

Citation

Structural basis for the Golgi association by the pleckstrin homology domain of the ceramide trafficking protein (CERT)
Sugiki, T., Takeuchi, K., Tokunaga, Y., Terasawa, H., Kumagai, K., Kawano, M., Nishijima, M., Hanada, K., Takahashi, H., Shimada, I.
J. Biol. Chem. (2012), 287, 33706-33718, PubMed 22869376 , DOI 10.1074/jbc.M112.367730 , Abstract

Related entities 1. CERT PH domain, : 1 : 5 : 2 : 194 entities Detail

Interaction partners 1. CERT PH domain, : 15 interactors Detail

Experiments performed 10 experiments Detail