BMRB: 1217

Purification and characterization of a non-reconstitutable azurin, obtained by heterologous expression of the Pseudomonas aeruginosa azu gene in Escherichia coli

Authors

van de Kamp, M., Hali, F.C., Rosato, N., Finazzi Agro, A., Canters, G.W.

Assembly

azurin

Entity

1. azurin (polymer), 128 monomers, 13945.66 Da Detail

AECSVDIQGN DQMQFNTNAI TVDKSCKQFT VNLSHPGNLP KNVMGHNWVL STAADMQGVV TDGMASGLDK DYLKPDDSRV IAHTKLIGSG EKDSVTFDVS KLKEGEQYMF FCTFPGHSAL MKGTLTLK

Formula weight

13945.66 Da

Source organism

Pseudomonas aeruginosa

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 9.4 %, Completeness: 5.2 %, Completeness (bb): 4.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	5.2 % (39 of 745)	5.2 % (39 of 745)
Backbone	4.2 % (11 of 263)	4.2 % (11 of 263)
Sidechain	5.8 % (28 of 482)	5.8 % (28 of 482)
Aromatic	13.5 % (7 of 52)	13.5 % (7 of 52)
Methyl	12.3 % (8 of 65)	12.3 % (8 of 65)

1. azurin

AECSVDIQGN DQMQFNTNAI TVDKSCKQFT VNLSHPGNLP KNVMGHNWVL STAADMQGVV TDGMASGLDK DYLKPDDSRV IAHTKLIGSG EKDSVTFDVS KLKEGEQYMF FCTFPGHSAL MKGTLTLK

Show sample condition

Sample

Temperature 298 K, pH 10

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Release date

1995-07-30

Citation

Purification and characterization of a non-reconstitutable azurin, obtained by heterologous expression of the Pseudomonas aeruginosa azu gene in Escherichia coli
van de Kamp, M., Hali, F.C., Rosato, N., Finazzi Agro, A., Canters, G.W.
Biochim. Biophys. Acta (1990), 1019, 283-292, PubMed 2119806 , DOI: , Abstract

Related entities 1. azurin, : 1 : 19 : 8 : 38 : 57 entities Detail

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail